Home LiteratureArticle Details

PMID: 9445473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Role of vesicle-associated syntaxin 5 in the assembly of pre-Golgi intermediates.

Science (New York, N.Y.) ·Vol. 279 ·No. 5351 ·1998-01-30 ·Pages 696-700

Rowe T, Dascher C, Bannykh S, Plutner H, Balch WE

Abstract

Syntaxins are thought to function during vesicular transport as receptors on the target membrane and to contribute to the specificity of membrane docking and fusion by interacting with vesicle-associated receptors. Here, syntaxin 5 (Syn5) was shown to be an integral component of endoplasmic reticulum-derived transport vesicles. This pool, but not the target, Golgi-associated Syn5 pool, was essential for the assembly of vesicular-tubular pre-Golgi intermediates and the delivery of cargo to the Golgi. The requirement for vesicle-associated Syn5 in transport suggests a reevaluation of the basis for operation of the early secretory pathway.

MeSH Terms

Adenosine Triphosphate/metabolism Animals Antibodies Biological Transport Carrier Proteins/metabolism Cell Line Endoplasmic Reticulum/metabolism Golgi Apparatus/metabolism,ultrastructure Mannose-Binding Lectins Membrane Fusion Membrane Glycoproteins Membrane Proteins/immunology,metabolism N-Ethylmaleimide-Sensitive Proteins Organelles/metabolism Qa-SNARE Proteins Qb-SNARE Proteins Qc-SNARE Proteins R-SNARE Proteins Rats SNARE Proteins Vesicular Transport Proteins Vesicular stomatitis Indiana virus/physiology Viral Envelope Proteins/metabolism

Chemicals

Antibodies BET1 protein, rat Carrier Proteins G protein, vesicular stomatitis virus Gosr2 protein, rat LMAN1 protein, human Mannose-Binding Lectins Membrane Glycoproteins Membrane Proteins Qa-SNARE Proteins Qb-SNARE Proteins Qc-SNARE Proteins R-SNARE Proteins SNARE Proteins Sec22a protein, rat Vesicular Transport Proteins Viral Envelope Proteins Adenosine Triphosphate N-Ethylmaleimide-Sensitive Proteins Nsf protein, rat

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Rowe T

Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Dascher C

Bannykh S

Plutner H

Balch W E

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.279.5351.696

Published

1998-01-30

Pages

696-700

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

NCI NIH HHS · CA58689 · United States

NIGMS NIH HHS · GM 42336 · United States

External Links

PubMed source DOI: 10.1126/science.279.5351.696 View journal details

Analysis Services

Role of vesicle-associated syntaxin 5 in the assembly of pre-Golgi intermediates.

Contact

WeChat Official Account