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PMID: 9445031 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

STP and Tip are essential for herpesvirus saimiri oncogenicity.

Journal of virology ·Vol. 72 ·No. 2 ·1998-02-00 ·Pages 1308-13

Duboise SM, Guo J, Czajak S, Desrosiers RC, Jung JU

Abstract

Mutant forms of herpesvirus saimiri (HVS) subgroup C strain 488 with deletions in either STP-C488 or Tip were constructed. The transforming potentials of the HVS mutants were tested in cell culture and in common marmosets. Parental HVS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, but neither of the deletion mutants produced such growth transformation. Wild-type HVS produced fatal lymphoma within 19 to 20 days of experimental infection of common marmosets, while HVS deltaSTP-C488 and HVS deltaTip were nononcogenic. Virus was repeatedly isolated from the peripheral blood of marmosets infected with mutant virus for more than 5 months. These results demonstrate that STP-C488 and Tip are not required for replication or persistence, but each is essential for transformation in cell culture and for lymphoma induction in common marmosets.

MeSH Terms
Animals Gene Deletion Genes, Viral Herpesviridae Infections/virology Herpesvirus 2, Saimiriine/genetics,pathogenicity Oncogene Proteins, Viral/genetics Phosphoproteins/genetics T-Lymphocytes/virology Tumor Virus Infections/virology Viral Proteins/genetics Virulence/genetics
Chemicals
Oncogene Proteins, Viral Phosphoproteins STP-C488 protein, Herpesvirus saimiri Viral Proteins tyrosine kinase interacting protein, Saimiriine herpesvirus 2
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Duboise S M
Department of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772-9102, USA.
Guo J
Czajak S
Desrosiers R C
Jung J U
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.72.2.1308-1313.1998
Published
1998-02-00
Pages
1308-13
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC124609
Subset
IM
Grants
NCRR NIH HHS · RR00168 · United States
NCRR NIH HHS · P51 RR000168 · United States
NCRR NIH HHS · K26 RR000168 · United States
NCI NIH HHS · CA31363 · United States
NCI NIH HHS · R01 CA031363 · United States
NIAID NIH HHS · AI38131 · United States
External Links
PubMed source DOI: 10.1128/JVI.72.2.1308-1313.1998 PMC Full Text View journal details
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