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PMID: 9421260 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Development-dependent inheritance of 5-azacytidine-induced epimutations in triticale: analysis of rDNA expression patterns.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology ·Vol. 5 ·No. 7 ·1997-11-00 ·Pages 445-50

Amado L, Abranches R, Neves N, Viegas W

Abstract

Genomic imprinting of rye origin rDNA sequences in triticale is modulated by DNA methylation responsible for ontogenic expression patterns of those sequences. Considering the dynamic nature of these phenomena, we evaluated the influence of plant development on the inheritance of modified rye rDNA expression patterns. DNA hypomethylation was induced in triticale by 5-azacytidine (5AC) treatments at distinct developmental stages of M1 plants, and expression patterns were analysed in M2. The activity of rye origin rRNA genes in progeny of untreated and 5AC-treated plants was evaluated by silver staining in meristematic root tip cells and in meiocytes at diplotene. In the progeny of 5AC-treated plants, a significant increase in rye rDNA expression was observed, contrasting with the residual activity in untreated plants. Significant differential effects of 5AC treatments were observed in M2 plants and correlated with the M1 plant developmental stage in which DNA hypomethylation was induced. Hypotheses to explain the origin of those differences are discussed here.

MeSH Terms
Antimetabolites, Antineoplastic/pharmacology Azacitidine/pharmacology Cell Nucleus/genetics,physiology Chromosomes/genetics DNA, Plant/genetics DNA, Ribosomal/genetics Edible Grain/genetics Gene Expression/genetics,physiology Gene Expression Regulation, Developmental Genome, Plant In Situ Hybridization Interphase Metaphase/genetics Mutation/drug effects,genetics Secale/genetics Silver Staining/standards
Chemicals
Antimetabolites, Antineoplastic DNA, Plant DNA, Ribosomal Azacitidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Amado L
Secção de Genética do Departamento de Botânica e Engenharia Biológica, Instituto Superior de Agronomia, Lisboa, Portugal. luisamado@isa.utl.pt
Abranches R
Neves N
Viegas W
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Article Info
Journal
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Abbr.
Chromosome Res
ISSN
0967-3849
DOI
10.1023/a:1018460828720
Email
luisamado@isa.utl.pt
Published
1997-11-00
Pages
445-50
Language
English
Region
Netherlands
NLM ID
9313452
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1018460828720 View journal details
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