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PMID: 9407035 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast.

Genes & development ·Vol. 11 ·No. 24 ·1997-12-15 ·Pages 3432-44

Stathopoulos AM, Cyert MS

Abstract

Calcineurin is a conserved Ca2+/calmodulin-dependent protein phosphatase that plays a critical role in Ca2+ signaling. We describe new components of a calcineurin-mediated response in yeast, the Ca2+-induced transcriptional activation of FKS2, which encodes a beta-1,3 glucan synthase. A 24-bp region of the FKS2 promoter was defined as sufficient to confer calcineurin-dependent transcriptional induction on a minimal promoter in response to Ca2+ and was named CDRE (for calcineurin-dependent response element). The product of CRZ1 (YNL027w) was identified as an activator of CDRE-driven transcription. Crz1p contains zinc finger motifs and binds specifically to the CDRE. Genetic analysis revealed that crz1Delta mutant cells exhibit several phenotypes similar to those of calcineurin mutants and that overexpression of CRZ1 in calcineurin mutants suppressed these phenotypes. These results suggest that Crz1p functions downstream of calcineurin to effect multiple calcineurin-dependent responses. Moreover, the calcineurin-dependent transcriptional induction of FKS2 in response to Ca2+, alpha-factor, and Na+ was found to require CRZ1. In addition, we found that the calcineurin-dependent transcriptional regulation of PMR2 and PMC1 required CRZ1. However, transcription of PMR2 and PMC1 was activated by only a subset of the treatments that activated FKS2 transcription. Thus, in response to multiple signals, calcineurin acts through the Crz1p transcription factor to differentially regulate the expression of several target genes in yeast.

MeSH Terms
Amino Acid Sequence Calcineurin/genetics,metabolism DNA-Binding Proteins Gene Expression Regulation, Fungal Genes, Reporter Glucosyltransferases Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutation Phenotype Plasmids/genetics Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Trans-Activators/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
CRZ1 protein, S cerevisiae DNA-Binding Proteins Membrane Proteins Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors Glucosyltransferases GSC2 protein, S cerevisiae Calcineurin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stathopoulos A M
Department of Biological Sciences, Stanford University, Stanford, California 94305-5020, USA.
Cyert M S
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.11.24.3432
Published
1997-12-15
Pages
3432-44
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316814
Subset
IM
Grants
NIGMS NIH HHS · 5T32GM07276-22 · United States
NIGMS NIH HHS · GM48729 · United States
External Links
PubMed source DOI: 10.1101/gad.11.24.3432 PMC Full Text View journal details
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