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PMID: 9405611 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 26 ·1997-12-23 ·Pages 14326-31

Rensing C, Mitra B, Rosen BP

Abstract

The first Zn(II)-translocating P-type ATPase has been identified as the product of o732, a potential gene identified in the sequencing of the Escherichia coli genome. This gene, termed zntA, was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain exhibited hypersensitivity to zinc and cadmium salts but not salts of other metals, suggesting a role in zinc homeostasis in E. coli. Everted membrane vesicles from a wild-type strain accumulated 65Zn(II) and 109Cd(II) by using ATP as an energy source. Transport was sensitive to vanadate, an inhibitor of P-type ATPases. Membrane vesicles from the zntA::kan strain did not accumulate those metal ions. Both the sensitive phenotype and transport defect of the mutant were complemented by expression of zntA on a plasmid.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Amino Acid Sequence Bacterial Proteins/genetics Cloning, Molecular Escherichia coli/genetics,metabolism Genes, Bacterial Molecular Sequence Data Sequence Alignment Zinc/metabolism
Chemicals
Bacterial Proteins Adenosine Triphosphatases Zn(II)-translocating P-type ATPase Zinc
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rensing C
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA.
Mitra B
Rosen B P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.26.14326
Published
1997-12-23
Pages
14326-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24962
Subset
IM
Grants
NIGMS NIH HHS · GM54102 · United States
NIGMS NIH HHS · R37 GM055425 · United States
NIGMS NIH HHS · F32 GM018973 · United States
NIGMS NIH HHS · GM55425 · United States
NIGMS NIH HHS · GM18973 · United States
NIGMS NIH HHS · R01 GM055425 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.26.14326 PMC Full Text View journal details
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