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PMID: 9398679 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells.

Molecular biology of the cell ·Vol. 8 ·No. 12 ·1997-12-00 ·Pages 2605-15

Sabry JH, Moores SL, Ryan S, Zang JH, Spudich JA

Abstract

Conventional myosin II plays a fundamental role in the process of cytokinesis where, in the form of bipolar thick filaments, it is thought to be the molecular motor that generates the force necessary to divide the cell. In Dictyostelium, the formation of thick filaments is regulated by the phosphorylation of three threonine residues in the tail region of the myosin heavy chain. We report here on the effects of this regulation on the localization of myosin in live cells undergoing cytokinesis. We imaged fusion proteins of the green-fluorescent protein with wild-type myosin and with myosins where the three critical threonines had been changed to either alanine or aspartic acid. We provide evidence that thick filament formation is required for the accumulation of myosin in the cleavage furrow and that if thick filaments are overproduced, this accumulation is markedly enhanced. This suggests that myosin localization in dividing cells is regulated by myosin heavy chain phosphorylation.

MeSH Terms
Actins/metabolism Amino Acid Substitution/genetics Animals Biological Transport Cell Division Cell Movement Cell Nucleus/metabolism Cell Survival Cells, Cultured Dictyostelium/cytology,genetics,metabolism Gene Deletion Green Fluorescent Proteins Kinetics Luminescent Proteins/chemistry,genetics,metabolism Myosin Heavy Chains/chemistry,genetics,metabolism Myosins/metabolism Phosphorylation Phosphothreonine/metabolism Recombinant Fusion Proteins/chemistry,genetics,metabolism Threonine/genetics,metabolism
Chemicals
Actins Luminescent Proteins Recombinant Fusion Proteins Phosphothreonine Green Fluorescent Proteins Threonine Myosin Heavy Chains Myosins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sabry J H
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA.
Moores S L
Ryan S
Zang J H
Spudich J A
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.8.12.2605
Published
1997-12-00
Pages
2605-15
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25731
Subset
IM
Grants
NIGMS NIH HHS · GM40509 · United States
External Links
PubMed source DOI: 10.1091/mbc.8.12.2605 PMC Full Text View journal details
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