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PMID: 9393687 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor sigmaB in Bacillus subtilis.

Journal of bacteriology ·Vol. 179 ·No. 23 ·1997-12-00 ·Pages 7251-6

Antelmann H, Engelmann S, Schmid R, Sorokin A, Lapidus A, Hecker M

Abstract

SigmaB-dependent general stress proteins (Gsps) of Bacillus subtilis are essential for the development of glucose-starvation-induced cross-resistance to oxidative challenge. However, the proteins directly involved in this nonspecific resistance to oxidative stress have to be identified. We found that one prominent Gsp displayed strong sequence similarity to the previously characterized oxidative-stress-inducible MrgA protein of B. subtilis and to the starvation-induced Dps/PexB protein of Escherichia coli. We therefore designated this prominent Gsp Dps. While MrgA belongs to the peroxide-stress-inducible proteins needed for the H2O2-inducible adaptive response to oxidative stress, Dps belongs to the proteins induced by heat, salt, or ethanol stress and after starvation for glucose but not by a sublethal oxidative challenge. Primer extension experiments identified two overlapping promoters upstream of the coding region of dps, one being sigmaB dependent (PB) and the other being sigmaB independent (P1). Both promoters contribute to the basal level of dps during growth. After stress or during entry into the stationary phase, transcription from PB strongly increased whereas transcription from P1 decreased. Mutant strains lacking Dps completely failed to develop glucose-starvation-induced resistance to oxidative stress. These results confirm our suggestion that sigmaB-dependent general stress proteins of B. subtilis are absolutely required for the development of nonspecific resistance to oxidative stress.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Bacterial Outer Membrane Proteins/biosynthesis,genetics Bacterial Proteins/biosynthesis,genetics,metabolism Base Sequence DNA-Binding Proteins/biosynthesis,genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Glucose/deficiency Molecular Sequence Data Oxidative Stress Sequence Analysis Sequence Homology, Amino Acid Sigma Factor/metabolism Transcription, Genetic
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins DNA-Binding Proteins DPS protein, Bacteria Escherichia coli Proteins MrgA protein, Bacillus subtilis SigB protein, Bacteria Sigma Factor dps protein, E coli Glucose
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Antelmann H
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Engelmann S
Schmid R
Sorokin A
Lapidus A
Hecker M
References (38)
38 references, click to expand
  1. Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function.
    J Bacteriol. 1987 Feb;169(2):771-8 PMID: 3027048
  2. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  3. Transcription factor sigma B of Bacillus subtilis controls a large stationary-phase regulon.
    J Bacteriol. 1993 Jul;175(13):3957-63 PMID: 8320211
  4. Genetic studies of a secondary RNA polymerase sigma factor in Bacillus subtilis.
    J Bacteriol. 1987 Aug;169(8):3464-9 PMID: 3112122
  5. Cloning, nucleotide sequence, and regulation of katE encoding a sigma B-dependent catalase in Bacillus subtilis.
    J Bacteriol. 1995 Oct;177(19):5598-605 PMID: 7559348
  6. Specific and general stress proteins in Bacillus subtilis--a two-deimensional protein electrophoresis study.
    Microbiology. 1997 Mar;143(Pt 3):999-1017 PMID: 9296790
  7. Characterization of the sigma 38-dependent expression of a core Escherichia coli starvation gene, pexB.
    J Bacteriol. 1994 Jul;176(13):3928-35 PMID: 8021175
  8. Relationship among oxidative stress, growth cycle, and sporulation in Bacillus subtilis.
    J Bacteriol. 1987 Dec;169(12):5771-5 PMID: 3119569
  9. A neomycin resistance gene cassette selectable in a single copy state in the Bacillus subtilis chromosome.
    Nucleic Acids Res. 1989 Jun 12;17(11):4410 PMID: 2500645
  10. Purification and characterization of a Synechococcus sp. strain PCC 7942 polypeptide structurally similar to the stress-induced Dps/PexB protein of Escherichia coli.
    Arch Microbiol. 1995 May;163(5):337-44 PMID: 7794101
  11. The molecular basis of carbon-starvation-induced general resistance in Escherichia coli.
    Mol Microbiol. 1991 Jan;5(1):3-10 PMID: 2014002
  12. Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions.
    Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8190-4 PMID: 7667267
  13. Heat-shock and general stress response in Bacillus subtilis.
    Mol Microbiol. 1996 Feb;19(3):417-28 PMID: 8830234
  14. Bacillus subtilis operon under the dual control of the general stress transcription factor sigma B and the sporulation transcription factor sigma H.
    Mol Microbiol. 1996 Apr;20(2):339-50 PMID: 8733232
  15. Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.
    J Bacteriol. 1992 May;174(10):3300-10 PMID: 1339421
  16. First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis.
    Electrophoresis. 1997 Aug;18(8):1451-63 PMID: 9298659
  17. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  18. The sigma B-dependent promoter of the Bacillus subtilis sigB operon is induced by heat shock.
    J Bacteriol. 1993 Apr;175(7):1929-35 PMID: 8458834
  19. A gene at 333 degrees on the Bacillus subtilis chromosome encodes the newly identified sigma B-dependent general stress protein GspA.
    J Bacteriol. 1995 Jun;177(12):3540-5 PMID: 7768864
  20. Metalloregulation in Bacillus subtilis: isolation and characterization of two genes differentially repressed by metal ions.
    J Bacteriol. 1993 Sep;175(17):5428-37 PMID: 8396117
  21. The oxidative stress response in Bacillus subtilis.
    FEMS Microbiol Lett. 1994 Dec 15;124(3):255-63 PMID: 7851732
  22. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  23. Temporal activation of beta-glucanase synthesis in Bacillus subtilis is mediated by the GTP pool.
    J Gen Microbiol. 1993 Sep;139(9):2041-5 PMID: 8245830
  24. Bacillus subtilis MrgA is a Dps(PexB) homologue: evidence for metalloregulation of an oxidative-stress gene.
    Mol Microbiol. 1995 Oct;18(2):295-300 PMID: 8709848
  25. Genetic analysis in Bacillus subtilis.
    Methods Enzymol. 1991;204:305-20 PMID: 1943780
  26. Treponema pallidum subspecies pallidum (Nichols) and Treponema pallidum subspecies pertenue (CDC 2575) differ in at least one nucleotide: comparison of two homologous antigens.
    Microb Pathog. 1989 Jan;6(1):29-42 PMID: 2471912
  27. Stress-induced activation of the sigma B transcription factor of Bacillus subtilis.
    J Bacteriol. 1993 Dec;175(24):7931-7 PMID: 8253681
  28. The role of the sigma factor sigma S (KatF) in bacterial global regulation.
    Annu Rev Microbiol. 1994;48:53-80 PMID: 7826018
  29. Similar organization of the sigB and spoIIA operons encoding alternate sigma factors of Bacillus subtilis RNA polymerase.
    J Bacteriol. 1990 Oct;172(10):5575-85 PMID: 2170324
  30. The dps promoter is activated by OxyR during growth and by IHF and sigma S in stationary phase.
    Mol Microbiol. 1994 Jul;13(2):265-72 PMID: 7984106
  31. Oxidative stress responses in Escherichia coli and Salmonella typhimurium.
    Microbiol Rev. 1991 Dec;55(4):561-85 PMID: 1779927
  32. Gene encoding the sigma 37 species of RNA polymerase sigma factor from Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5943-7 PMID: 3016731
  33. A novel DNA-binding protein with regulatory and protective roles in starved Escherichia coli.
    Genes Dev. 1992 Dec;6(12B):2646-54 PMID: 1340475
  34. Simultaneous and rapid isolation of bacterial and eukaryotic DNA and RNA: a new approach for isolating DNA.
    Biotechniques. 1991 Jul;11(1):94-101 PMID: 1720004
  35. Analysis of the induction of general stress proteins of Bacillus subtilis.
    Microbiology. 1994 Apr;140 ( Pt 4):741-52 PMID: 8012595
  36. Oxidative stress and growth temperature in Bacillus subtilis.
    J Bacteriol. 1987 Dec;169(12):5766-70 PMID: 3119568
  37. Impaired oxidative stress resistance of Bacillus subtilis sigB mutants and the role of katA and katE.
    FEMS Microbiol Lett. 1996 Nov 15;145(1):63-9 PMID: 8931328
  38. Molecular cloning and sequencing of a non-haem bromoperoxidase gene from Streptomyces aureofaciens ATCC 10762.
    J Gen Microbiol. 1992 Jun;138(6):1123-31 PMID: 1527491
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.23.7251-7256.1997
Published
1997-12-00
Pages
7251-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179673
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.179.23.7251-7256.1997 PMC Full Text View journal details
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