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PMID: 9389651 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses.

Genes & development ·Vol. 11 ·No. 23 ·1997-12-01 ·Pages 3194-205

Peng J, Carol P, Richards DE, King KE, Cowling RJ, Murphy GP, Harberd NP

Abstract

The Arabidopsis gai mutant allele confers a reduction in gibberellin (GA) responsiveness. Here we report the molecular cloning of GAI and a closely related gene GRS. The predicted GAI (wild-type) and gai (mutant) proteins differ only by the deletion of a 17-amino-acid segment from within the amino-terminal region. GAI and GRS contain nuclear localization signals, a region of homology to a putative transcription factor, and motifs characteristic of transcriptional coactivators. Genetic analysis indicates that GAI is a repressor of GA responses, that GA can release this repression, and that gai is a mutant repressor that is relatively resistant to the effects of GA. Mutations at SPY and GAR2 suppress the gai phenotype, indicating the involvement of GAI, SPY, and GAR2 in a signaling pathway that regulates GA responses negatively. The existence of this pathway suggests that GA modulates plant growth through derepression rather than through simple stimulation.

MeSH Terms
Alleles Amino Acid Sequence Arabidopsis/drug effects,genetics,growth & development Arabidopsis Proteins Base Sequence Cloning, Molecular Consensus Sequence DNA, Plant Genes, Plant Gibberellins/antagonists & inhibitors,pharmacology Mixed Function Oxygenases/genetics Molecular Sequence Data Mutagenesis, Insertional Nuclear Localization Signals/genetics Open Reading Frames Plant Growth Regulators/pharmacology Plant Proteins/genetics,metabolism RNA, Plant/metabolism Sequence Homology, Amino Acid Signal Transduction Suppression, Genetic Triazoles/pharmacology
Chemicals
Arabidopsis Proteins DNA, Plant GAI protein, Arabidopsis GRS protein, Arabidopsis Gibberellins Nuclear Localization Signals Plant Growth Regulators Plant Proteins RNA, Plant Triazoles paclobutrazol Mixed Function Oxygenases gibberellin, 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating, oxidizing)
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Peng J
Department of Molecular Genetics, John Innes Centre, Norwich Research Park, Norwich NR4 7UJ, UK.
Carol P
Richards D E
King K E
Cowling R J
Murphy G P
Harberd N P
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.11.23.3194
Published
1997-12-01
Pages
3194-205
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316750
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BBS/E/J/00000583 · United Kingdom
Databases
GENBANK
Y15193, Y15194
External Links
PubMed source DOI: 10.1101/gad.11.23.3194 PMC Full Text View journal details
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