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PMID: 9376675 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids.

Glycobiology ·Vol. 7 ·No. 6 ·1997-09-00 ·Pages 731-5

Agusti R, Couto AS, Campetella OE, Frasch AC, de Lederkremer RM

Abstract

The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. cruzi was made.

MeSH Terms

Animals Chromatography, Thin Layer Glycosylphosphatidylinositols/metabolism Membrane Lipids/metabolism Mice Neuraminidase/metabolism Phosphatidylinositol Diacylglycerol-Lyase Phosphoinositide Phospholipase C Substrate Specificity Trypanosoma cruzi/enzymology Type C Phospholipases/metabolism

Chemicals

Glycosylphosphatidylinositols Membrane Lipids Type C Phospholipases Phosphoinositide Phospholipase C Neuraminidase Phosphatidylinositol Diacylglycerol-Lyase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Agusti R

CIHIDECAR, Departmento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.

Couto A S

Campetella O E

Frasch A C

de Lederkremer R M

Article Info

Journal

Glycobiology

Abbr.

Glycobiology

ISSN

0959-6658

DOI

10.1093/glycob/7.6.731

Published

1997-09-00

Pages

731-5

Language

English

Region

England

NLM ID

9104124

Subset

External Links

PubMed source DOI: 10.1093/glycob/7.6.731 View journal details

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The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids.

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