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PMID: 9371605 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The herpes simplex virus virulence factor ICP34.5 and the cellular protein MyD116 complex with proliferating cell nuclear antigen through the 63-amino-acid domain conserved in ICP34.5, MyD116, and GADD34.

Journal of virology ·Vol. 71 ·No. 12 ·1997-12-00 ·Pages 9442-9

Brown SM, MacLean AR, McKie EA, Harland J

Abstract

The herpes simplex virus (HSV) virulence factor ICP34.5, the mouse myeloid differentiation protein MyD116, and the hamster growth arrest and DNA damage protein GADD34 share a 63-amino-acid carboxyl domain which has significant homologies to otherwise divergent proteins. Here we report that both ICP34.5 and its cellular homolog MyD116 complex through the conserved domain with proliferating cell nuclear antigen. In addition, HSV infection induces a novel 70-kDa cellular protein detectable by antisera to both ICP34.5 and GADD34, demonstrating that this novel protein possesses homology with the 63-amino-acid conserved domain.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals Antibodies/immunology Antigens, Differentiation Binding Sites Cell Cycle Proteins Cell Line Conserved Sequence Cricetinae DNA Damage Gene Expression Glutathione Transferase Herpesvirus 1, Human/pathogenicity Humans Mice Neoplasm Proteins Proliferating Cell Nuclear Antigen/metabolism Protein Phosphatase 1 Proteins/genetics,immunology,metabolism RNA Rabbits Recombinant Fusion Proteins/genetics Transcription, Genetic Tumor Cells, Cultured Viral Proteins/genetics,immunology,metabolism Virulence
Chemicals
Antibodies Antigens, Differentiation Cell Cycle Proteins Myd116 protein, mouse Neoplasm Proteins Proliferating Cell Nuclear Antigen Proteins Recombinant Fusion Proteins Viral Proteins gamma 34.5 protein, Human herpesvirus 1 RNA Glutathione Transferase PPP1R15A protein, human Ppp1r15a protein, mouse Protein Phosphatase 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brown S M
Glasgow University Neurovirology Research Laboratories, Institute of Neurological Sciences, Southern General Hospital, United Kingdom. smbrown@clinmed.gla.ac.uk
MacLean A R
McKie E A
Harland J
References (45)
45 references, click to expand
  1. The carboxyl terminus of the murine MyD116 gene substitutes for the corresponding domain of the gamma(1)34.5 gene of herpes simplex virus to preclude the premature shutoff of total protein synthesis in infected human cells.
    J Virol. 1996 Jan;70(1):84-90 PMID: 8523596
  2. Therapy of experimental human brain tumors using a neuroattenuated herpes simplex virus mutant.
    Lab Invest. 1995 Nov;73(5):636-48 PMID: 7474937
  3. Selective vulnerability of mouse CNS neurons to latent infection with a neuroattenuated herpes simplex virus-1.
    J Neurosci. 1996 Sep 15;16(18):5644-53 PMID: 8795620
  4. The range and distribution of murine central nervous system cells infected with the gamma(1)34.5- mutant of herpes simplex virus 1.
    J Virol. 1997 Jul;71(7):5560-9 PMID: 9188630
  5. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence.
    Virology. 1962 Feb;16:147-51 PMID: 14468055
  6. Temperature-sensitive mutants of herpes simplex virus type 2.
    J Gen Virol. 1971 Nov;13(2):373-6 PMID: 4333719
  7. Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map.
    J Gen Virol. 1973 Mar;18(3):329-46 PMID: 4348796
  8. Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild type and sixteen temperature-sensitive mutants of HSV strain 17.
    J Gen Virol. 1976 Jun;31(3):347-72 PMID: 180249
  9. Isolation and characterization of deletion mutants of herpes simplex virus type 2 (strain HG52).
    J Gen Virol. 1985 Jun;66 ( Pt 6):1305-21 PMID: 2989417
  10. The terminal a sequence of the herpes simplex virus genome contains the promoter of a gene located in the repeat sequences of the L component.
    J Virol. 1986 Feb;57(2):629-37 PMID: 3003394
  11. Identification by antibody to a synthetic peptide of a protein specified by a diploid gene located in the terminal repeats of the L component of herpes simplex virus genome.
    J Virol. 1986 Jun;58(3):843-50 PMID: 3009891
  12. Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta.
    Nature. 1987 Apr 2-8;326(6112):515-7 PMID: 2882423
  13. Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxiliary protein.
    Nature. 1987 Apr 2-8;326(6112):517-20 PMID: 2882424
  14. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  15. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  16. A variant of herpes simplex virus type 2 strain HG52 with a 1.5 kb deletion in RL between 0 to 0.02 and 0.81 to 0.83 map units is non-neurovirulent for mice.
    J Gen Virol. 1989 Mar;70 ( Pt 3):705-16 PMID: 2543755
  17. The herpes simplex virus type 2 (HG52) variant JH2604 has a 1488 bp deletion which eliminates neurovirulence in mice.
    J Gen Virol. 1989 Nov;70 ( Pt 11):3073-8 PMID: 2555437
  18. Mammalian genes coordinately regulated by growth arrest signals and DNA-damaging agents.
    Mol Cell Biol. 1989 Oct;9(10):4196-203 PMID: 2573827
  19. Complexity of the immediate early response of myeloid cells to terminal differentiation and growth arrest includes ICAM-1, Jun-B and histone variants.
    Oncogene. 1990 Mar;5(3):387-96 PMID: 1690380
  20. Mapping of herpes simplex virus-1 neurovirulence to gamma 134.5, a gene nonessential for growth in culture.
    Science. 1990 Nov 30;250(4985):1262-6 PMID: 2173860
  21. Inactivation of the shutoff gene (UL41) of herpes simplex virus types 1 and 2.
    J Gen Virol. 1990 Dec;71 ( Pt 12):2961-7 PMID: 2177088
  22. Herpes simplex virus type 1 deletion variants 1714 and 1716 pinpoint neurovirulence-related sequences in Glasgow strain 17+ between immediate early gene 1 and the 'a' sequence.
    J Gen Virol. 1991 Mar;72 ( Pt 3):631-9 PMID: 1848598
  23. Investigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins.
    J Gen Virol. 1991 Apr;72 ( Pt 4):897-906 PMID: 1849972
  24. Neurovirulence factor.
    Nature. 1991 Oct 17;353(6345):609 PMID: 1656275
  25. The gamma 1(34.5) gene of herpes simplex virus 1 precludes neuroblastoma cells from triggering total shutoff of protein synthesis characteristic of programed cell death in neuronal cells.
    Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3266-70 PMID: 1314384
  26. Proliferating cell nuclear antigen is required for DNA excision repair.
    Cell. 1992 Apr 17;69(2):367-74 PMID: 1348971
  27. The timing of the formation and usage of replicase clusters in S-phase nuclei of human diploid fibroblasts.
    J Cell Sci. 1991 Dec;100 ( Pt 4):869-76 PMID: 1687687
  28. Peripheral replication and latency reactivation kinetics of the non-neurovirulent herpes simplex virus type 1 variant 1716.
    J Gen Virol. 1992 Apr;73 ( Pt 4):967-70 PMID: 1321881
  29. Status of the ICP34.5 gene in herpes simplex virus type 1 strain 17.
    J Gen Virol. 1992 Apr;73 ( Pt 4):971-3 PMID: 1321882
  30. The herpes simplex virus type 1 strain 17 open reading frame RL1 encodes a polypeptide of apparent M(r) 37K equivalent to ICP34.5 of herpes simplex virus type 1 strain F.
    J Gen Virol. 1993 Nov;74 ( Pt 11):2493-7 PMID: 8245868
  31. The gadd and MyD genes define a novel set of mammalian genes encoding acidic proteins that synergistically suppress cell growth.
    Mol Cell Biol. 1994 Apr;14(4):2361-71 PMID: 8139541
  32. Characterization of the herpes simplex virus type 1 strain 17+ neurovirulence gene RL1 and its expression in a bacterial system.
    J Gen Virol. 1994 Apr;75 ( Pt 4):733-41 PMID: 8151292
  33. Herpes simplex virus type 1 immediate-early protein Vmw110 binds strongly and specifically to a 135-kDa cellular protein.
    Virology. 1994 May 1;200(2):457-69 PMID: 8178435
  34. The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA.
    Nature. 1994 Jun 16;369(6481):574-8 PMID: 7911228
  35. Herpes simplex virus 1 gamma(1)34.5 gene function, which blocks the host response to infection, maps in the homologous domain of the genes expressed during growth arrest and DNA damage.
    Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5247-51 PMID: 8202476
  36. Cell type and cell state determine differential in vitro growth of non-neurovirulent ICP34.5-negative herpes simplex virus types 1 and 2.
    J Gen Virol. 1994 Sep;75 ( Pt 9):2367-77 PMID: 8077935
  37. Cdk-interacting protein 1 directly binds with proliferating cell nuclear antigen and inhibits DNA replication catalyzed by the DNA polymerase delta holoenzyme.
    Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8655-9 PMID: 7915843
  38. Differential effects by the p21 CDK inhibitor on PCNA-dependent DNA replication and repair.
    Nature. 1994 Oct 6;371(6497):534-7 PMID: 7935768
  39. ICP34.5 influences herpes simplex virus type 1 maturation and egress from infected cells in vitro.
    J Gen Virol. 1994 Dec;75 ( Pt 12):3679-86 PMID: 7996163
  40. Replication, establishment of latent infection, expression of the latency-associated transcripts and explant reactivation of herpes simplex virus type 1 gamma 34.5 mutants in a mouse eye model.
    J Gen Virol. 1995 Feb;76 ( Pt 2):321-32 PMID: 7844554
  41. A small peptide inhibitor of DNA replication defines the site of interaction between the cyclin-dependent kinase inhibitor p21WAF1 and proliferating cell nuclear antigen.
    Curr Biol. 1995 Mar 1;5(3):275-82 PMID: 7780738
  42. Treatment of experimental intracranial murine melanoma with a neuroattenuated herpes simplex virus 1 mutant.
    Virology. 1995 Aug 1;211(1):94-101 PMID: 7645240
  43. Association of a M(r) 90,000 phosphoprotein with protein kinase PKR in cells exhibiting enhanced phosphorylation of translation initiation factor eIF-2 alpha and premature shutoff of protein synthesis after infection with gamma 134.5- mutants of herpes simplex virus 1.
    Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10516-20 PMID: 7479831
  44. Direct interaction of Gadd45 with PCNA and evidence for competitive interaction of Gadd45 and p21Waf1/Cip1 with PCNA.
    Oncogene. 1995 Nov 16;11(10):1931-7 PMID: 7478510
  45. Selective in vitro replication of herpes simplex virus type 1 (HSV-1) ICP34.5 null mutants in primary human CNS tumours--evaluation of a potentially effective clinical therapy.
    Br J Cancer. 1996 Sep;74(5):745-52 PMID: 8795577
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.12.9442-9449.1997
Email
smbrown@clinmed.gla.ac.uk
Published
1997-12-00
Pages
9442-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC230249
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.71.12.9442-9449.1997 PMC Full Text View journal details
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