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PMID: 9370470 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Myoglobin and hemoglobin rotational diffusion in the cell.

Biophysical journal ·Vol. 73 ·No. 5 ·1997-11-00 ·Pages 2764-70

Wang D, Kreutzer U, Chung Y, Jue T

Abstract

The detection of the 1H NMR signal of myoglobin (Mb) in tissue opens an opportunity to examine its cellular diffusion property, which is central to its purported role in facilitating oxygen transport. In perfused myocardium the field-dependent transverse relaxation analysis of the deoxy Mb proximal histidyl NdeltaH indicates that the Mb rotational correlation time in the cell is only approximately 1.4 times longer than it is in solution. Such a mobility is consistent with the theory that Mb facilitates oxygen diffusion from the sarcoplasm to the mitochondria. The microviscosities of the erythrocyte and myocyte environment are different. The hemoglobin (Hb) rotational correlation time is 2.2 longer in the cell than in solution. Because both the overlapping Hb and Mb signals are visible in vivo, a relaxation-based NMR strategy has been developed to discriminate between them.

MeSH Terms
Animals Diffusion Erythrocytes/chemistry,metabolism Hemoglobins/analysis,chemistry,metabolism Histidine/chemistry,metabolism Horses Humans Myocardium/chemistry,metabolism Myoglobin/analysis,chemistry,metabolism Nuclear Magnetic Resonance, Biomolecular Oxygen/metabolism Viscosity
Chemicals
Hemoglobins Myoglobin Histidine deoxyhemoglobin Oxygen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wang D
Biological Chemistry Department, University of California Davis, 95616-8635, USA.
Kreutzer U
Chung Y
Jue T
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31 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(97)78305-X
Published
1997-11-00
Pages
2764-70
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1181178
Subset
IM
Grants
NIGMS NIH HHS · GM44916 · United States
NHLBI NIH HHS · HL09274 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(97)78305-X PMC Full Text View journal details
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