Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles.

Emerging infectious diseases ·Vol. 3 ·No. 4 ·1997-00-00 ·Pages 567-73

Peng MM, Xiao L, Freeman AR, Arrowood MJ, Escalante AA, Weltman AC, Ong CS, Mac Kenzie WR, Lal AA, Beard CB

Abstract

We report the results of molecular analysis of 39 isolates of Cryptosporidium parvum from human and bovine sources in nine human outbreaks and from bovine sources from a wide geographic distribution. All 39 isolates could be divided into either of two genotypes, on the basis of genetic polymorphism observed at the thrombospondin-related adhesion protein (TRAP-C2) locus. Genotype 1 was observed only in isolates from humans. Genotype 2, however, was seen in calf isolates and in isolates from a subset of human patients who reported direct exposure to infected cattle or consumed items thought to be contaminated with cattle faces. Furthermore, experimental infection studies showed that genotype 2 isolates were infective to mice or calves under routine laboratory conditions, whereas genotype 1 isolates were not. These results support the occurrence of two distinct transmission cycles of C. parvum in humans.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cattle Cryptosporidium parvum/classification,genetics DNA, Protozoan/analysis,chemistry Genotype Humans Mice Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic

Chemicals

DNA, Protozoan

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Peng M M

University of Michigan, Ann Arbor, USA.

Xiao L

Freeman A R

Arrowood M J

Escalante A A

Weltman A C

Ong C S

Mac Kenzie W R

Lal A A

Beard C B

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Article Info

Journal

Emerging infectious diseases

Abbr.

Emerg Infect Dis

ISSN

1080-6040

DOI

10.3201/eid0304.970423

Published

1997-00-00

Pages

567-73

Language

English

Region

United States

NLM ID

9508155

PMCID

PMC2640093

Subset

External Links

PubMed source DOI: 10.3201/eid0304.970423 PMC Full Text View journal details

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Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles.

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