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PMID: 9358753 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Serial transplantation reveals the stem-cell-like regenerative potential of adult mouse hepatocytes.

The American journal of pathology ·Vol. 151 ·No. 5 ·1997-11-00 ·Pages 1273-80

Overturf K, al-Dhalimy M, Ou CN, Finegold M, Grompe M

Abstract

Previous work has shown that adult mouse hepatocytes can divide at least 18 times in vivo. To test whether this represents the upper limit of their regenerative capacity, we performed serial transplantation of hepatocytes in the fumarylacetoacetate hydrolase deficiency murine model of liver repopulation. Hepatocytes from adult donors were serially transplanted in limiting numbers six times and resulted in complete repopulation during each cycle. This corresponds to a minimal number of 69 cell doublings or a 7.3 x 10(20)-fold expansion. No evidence for abnormal liver function or altered hepatic architecture was found in repopulated animals. We conclude that a fraction of adult mouse hepatocytes have growth potential similar to that of hematopoietic stem cells.

MeSH Terms
Animals Cell Transformation, Neoplastic Cell Transplantation Liver/cytology,physiology Liver Regeneration/physiology Mice Mice, Mutant Strains Stem Cells/physiology Tissue Donors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Overturf K
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201, USA.
al-Dhalimy M
Ou C N
Finegold M
Grompe M
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1997-11-00
Pages
1273-80
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1858091
Subset
IM
Grants
NIDDK NIH HHS · DK 48252 · United States
NIDDK NIH HHS · DK009429 · United States
External Links
PubMed source PMC Full Text View journal details
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