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PMID: 9358744 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mice lacking the thrombin receptor, PAR1, have normal skin wound healing.

The American journal of pathology ·Vol. 151 ·No. 5 ·1997-11-00 ·Pages 1199-204

Connolly AJ, Suh DY, Hunt TK, Coughlin SR

Abstract

Thrombin's actions on platelets, macrophages, fibroblasts, and endothelial cells have prompted the hypothesis that thrombin may be important for inflammatory and fibroproliferative processes in wound healing. Protease-activated receptor 1 (PAR1) is a G-protein-coupled receptor that mediates many of the cellular activities of thrombin. To test the role of this receptor in vivo, we generated PAR1-deficient mice. Despite the observation that fibroblasts cultured from these mice lacked responsiveness to thrombin in vitro, we now report that there was no difference detected between wild-type and PAR1-deficient mice in skin wound healing assays including time to closure of open wounds, tensile strength of healed incisional wounds, wound histology, and hydroxyproline/DNA content of wound implants. We conclude that PAR1 is not necessary for normal skin wound healing in mice.

MeSH Terms
Animals Male Mice Mice, Inbred C57BL Mice, Inbred Strains Receptor, PAR-1 Receptors, Thrombin/deficiency,physiology Reference Values Skin/injuries,pathology Time Factors Wound Healing/physiology Wounds, Penetrating/metabolism,pathology,physiopathology
Chemicals
Receptor, PAR-1 Receptors, Thrombin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Connolly A J
Department of Pathology, University of California, San Francisco 94143-0130, USA.
Suh D Y
Hunt T K
Coughlin S R
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1997-11-00
Pages
1199-204
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1858088
Subset
IM
Grants
NIDDK NIH HHS · DK50267 · United States
NIGMS NIH HHS · GM27345 · United States
NHLBI NIH HHS · HL03234 · United States
External Links
PubMed source PMC Full Text View journal details
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