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PMID: 9343257 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cross-clade human immunodeficiency virus (HIV)-specific cytotoxic T-lymphocyte responses in HIV-infected Zambians.

Journal of virology ·Vol. 71 ·No. 11 ·1997-11-00 ·Pages 8908-11

Betts MR, Krowka J, Santamaria C, Balsamo K, Gao F, Mulundu G, Luo C, N'Gandu N, Sheppard H, Hahn BH, Allen S, Frelinger JA

Abstract

We have examined cross-clade HIV-specific cytotoxic T-lymphocyte (CTL) activity in peripheral blood of eight Zambian individuals infected with non-B-clade human immunodeficiency virus type 1 (HIV-1). Heteroduplex mobility assay and partial sequence analysis of env and gag genes strongly suggests that all the HIV-infected subjects were infected with clade C HIV-1. Six of eight C-clade HIV-infected individuals elicited CTL activity specific for recombinant vaccinia virus-infected autologous targets expressing HIV gag-pol-env derived from B-clade HIV-1 (IIIB). Recognition of individual recombinant HIV-1 B-clade vaccinia virus-infected targets expressing gag, pol, or env was variable among the patients tested, indicating that cross-clade CTL activity is not limited to a single HIV protein. These data demonstrate that HIV clade C-infected individuals can mount vigorous HIV clade B-reactive CTL responses.

MeSH Terms
Cross Reactions DNA, Viral/genetics HIV Antigens/immunology HIV Infections/immunology,virology HIV-1/immunology Humans Immunity, Cellular Phylogeny T-Lymphocytes, Cytotoxic/immunology United States Zambia
Chemicals
DNA, Viral HIV Antigens
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Betts M R
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, 27599-7290, USA.
Krowka J
Santamaria C
Balsamo K
Gao F
Mulundu G
Luo C
N'Gandu N
Sheppard H
Hahn B H
Allen S
Frelinger J A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.11.8908-8911.1997
Published
1997-11-00
Pages
8908-11
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC192363
Subset
IM
Grants
NIAID NIH HHS · R01-AI-29324 · United States
NIAID NIH HHS · R01-AI-35170 · United States
NIAID NIH HHS · R01-AI-82515 · United States
External Links
PubMed source DOI: 10.1128/JVI.71.11.8908-8911.1997 PMC Full Text View journal details
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