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PMID: 9342335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular switch in signal transduction: reaction paths of the conformational changes in ras p21.

Ma J, Karplus M

Abstract

Conformational changes in ras p21 triggered by the hydrolysis of GTP play an essential role in the signal transduction pathway. The path for the conformational change is determined by molecular dynamics simulation with a holonomic constraint directing the system from the known GTP-bound structure (with the gamma-phosphate removed) to the GDP-bound structure. The simulation is done with a shell of water molecules surrounding the protein. In the switch I region, the side chain of Tyr-32, which undergoes a large displacement, moves through the space between loop 2 and the rest of the protein, rather than on the outside of the protein. As a result, the charged residues Glu-31 and Asp-33, which interact with Raf in the homologous RafRBD-Raps complex, remain exposed during the transition. In the switch II region, the conformational changes of alpha2 and loop 4 are strongly coupled. A transient hydrogen bonding complex between Arg-68 and Tyr-71 in the switch II region and Glu-37 in switch I region stabilizes the intermediate conformation of alpha2 and facilitates the unwinding of a helical turn of alpha2 (residues 66-69), which in turn permits the larger scale motion of loop 4. Hydrogen bond exchange between the protein and solvent molecules is found to be important in the transition. Possible functional implications of the results are discussed.

MeSH Terms
Computer Simulation Guanosine Diphosphate/metabolism Guanosine Triphosphate/metabolism Models, Molecular Protein Conformation Protein Folding Proto-Oncogene Proteins p21(ras)/chemistry Signal Transduction
Chemicals
Guanosine Diphosphate Guanosine Triphosphate Proto-Oncogene Proteins p21(ras)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ma J
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
Karplus M
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1997-10-28
Pages
11905-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23651
Subset
IM
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