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PMID: 9338969 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Coexpression of the maize delta-zein and beta-zein genes results in stable accumulation of delta-zein in endoplasmic reticulum-derived protein bodies formed by beta-zein.

The Plant cell ·Vol. 9 ·No. 9 ·1997-09-00 ·Pages 1683-96

Bagga S, Adams HP, Rodriguez FD, Kemp JD, Sengupta-Gopalan C

Abstract

Zeins, the major seed storage proteins of maize, are of four distinct types: alpha, beta, delta, and gamma. They are synthesized on the rough endoplasmic reticulum (ER) in a sequential manner and deposited in ER-derived protein bodies. We investigated the potential for producing sulfur-rich beta-zein and delta-zein proteins in leaf and seed tissues by expressing the corresponding genes in a constitutive manner in transgenic tobacco. The delta-zein and beta-zein, when synthesized individually, were stable in the vegetative tissues and were deposited in unique, zein-specific ER-derived protein bodies. Coexpression of delta-zein and beta-zein genes, however, showed that delta-zein was colocalized in beta-zein-containing protein bodies and that the level of delta-zein was fivefold higher in delta-/beta-zein plants than in delta-zein plants. We conclude that delta-zein interacts with beta-zein and that the interaction has a stabilizing effect on delta-zein.

MeSH Terms

Endoplasmic Reticulum/metabolism Microscopy, Immunoelectron Plants, Genetically Modified Plants, Toxic Tobacco/genetics,ultrastructure Zea mays/genetics,metabolism Zein/genetics

Chemicals

Zein

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Bagga S

Agronomy and Horticulture Department, New Mexico State University, Las Cruces 88003, USA.

Adams H P

Rodriguez F D

Kemp J D

Sengupta-Gopalan C

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Article Info

Journal

The Plant cell

Abbr.

Plant Cell

ISSN

1040-4651

DOI

10.1105/tpc.9.9.1683

Published

1997-09-00

Pages

1683-96

Language

English

Region

England

NLM ID

9208688

PMCID

PMC157043

Subset

Grants

NIGMS NIH HHS · GMO7767 · United States

External Links

PubMed source DOI: 10.1105/tpc.9.9.1683 PMC Full Text View journal details

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Coexpression of the maize delta-zein and beta-zein genes results in stable accumulation of delta-zein in endoplasmic reticulum-derived protein bodies formed by beta-zein.

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