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题目:: Localization of microtubules containing posttranslationally modified tubulin in cochlear epithelial cells during development.
作者:: Tannenbaum(J),Slepecky(N B)
状态:: 发布时间1997-11-24 , 更新时间 2006-11-15
期刊:: Cell Motil Cytoskeleton
摘要:: In the adult gerbil inner ear, hair cell microtubules contain predominantly tyrosinated tubulin while supporting cell microtubules contain almost exclusively other isoforms. This cell-type specific segregation of tubulin isoforms is unusual, and in this respect the sensory and supporting cells in this sensory organ differ from other cells observed both in vivo and in vitro. Thus, we hypothesized there must be a shift in the presence and location of tubulin isoforms during development, directly associated with the onset of specialized functions of the cells. We describe the appearance and/or disappearance of tubulin isoforms in sensory hair cells and five different supporting cells (inner and outer pillar cells, Deiters cells, cells of Kölliker's organ, and cells of the tympanic covering layer) during development of the gerbil organ of Corti from birth to 14 days after birth. Tyrosinated tubulin was initially present in all cells and remained predominant in cells that decrease in number (Kölliker's organ and tympanic covering layer) and exhibit active processes such as secretion and motility (sensory cells). Posttranslational modifications occurred in the supporting cells in a time-dependent manner as the number and length of microtubules increased and development proceeded, but the establishment of elongated cell shape and polarity occurred prior to the appearance of acetylation, detyrosination, and polyglutamylation of tubulin. In the pillar and Deiters cells, posttranslational modifications progressed from cell apex to base in the same direction as microtubule elongation. In the pillar cells, posttranslational modifications occurred first at the apical surfaces. In the pillar cells, the appearance of acetylated tubulin was rapidly followed by the appearance of detyrosinated tubulin. In Deiters cells, the appearance of acetylated tubulin preceded the appearance of detyrosinated tubulin by one or more days. At onset of cochlear function, detyrosinated tubulin and acetylated tubulin had achieved their adult-like pattern, but polyglutamylated tubulin had not.
语言:: eng
DOI:: 10.1002/(SICI)1097-0169(1997)38:2<146::AID-CM4>3.0.CO;2-5

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