Abstract
It has been assumed that constitutive and regulated splicing of RNA polymerase II transcripts depends exclusively on signals present in the RNA molecule. Here we show that changes in promoter structure strongly affect splice site selection. We investigated the splicing of the ED I exon, which encodes a facultative type III repeat of fibronectin, whose inclusion is regulated during development and in proliferative processes. We used an alternative splicing assay combined with promoter swapping to demonstrate that the extent of ED I splicing is dependent on the promoter structure from which the transcript originated and that this regulation is independent of the promoter strength. Thus, these results provide the first evidence for coupling between alternative splicing and promoter-specific transcription, which agrees with recent cytological and biochemical evidence of coordination between splicing and transcription.
MeSH Terms
Alternative Splicing
Exons
Fibronectins/biosynthesis,genetics
Globins/biosynthesis
Humans
Models, Genetic
Polymerase Chain Reaction
Promoter Regions, Genetic
RNA Polymerase II/metabolism
RNA Precursors/metabolism
RNA, Messenger/biosynthesis
Recombinant Fusion Proteins/biosynthesis
Transcription, Genetic
Transfection
Tumor Cells, Cultured
beta-Galactosidase/biosynthesis
Chemicals
Fibronectins
RNA Precursors
RNA, Messenger
Recombinant Fusion Proteins
Globins
RNA Polymerase II
beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cramer P
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and Instituto de Investigaciones en Ingenier-ia Gen-etica y Biolog-ia Molecular, Ciudad Universitaria, Buenos Aires, Argentina.
Pesce C G
Baralle F E
Kornblihtt A R
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