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PMID: 9321397 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cleavage of rabaptin-5 blocks endosome fusion during apoptosis.

The EMBO journal ·Vol. 16 ·No. 20 ·1997-10-15 ·Pages 6182-91

Cosulich SC, Horiuchi H, Zerial M, Clarke PR, Woodman PG

Abstract

Cells undergoing apoptosis exhibit striking changes in membrane organization, including plasma membrane blebbing and invagination, vacuolation and fragmentation of organelles, and alterations in the surface expression of receptors. The underlying mechanisms for these changes are unknown, though alterations in vesicular fusion are likely to play a role. Using a cell-free system based on Xenopus laevis egg extracts we have found that endosome fusion is blocked during apoptosis. Inhibition of fusion is prevented by Bcl-2 or Bcl-xL, two negative regulators of apoptosis, or by specific inhibitors of members of the caspase family of apoptotic proteases. Selective cleavage of Rabaptin-5, an essential and rate-limiting component of endosome fusion, is responsible for the loss of fusion activity. Cleavage of Rabaptin-5 also occurs in cellular models for apoptosis. These results suggest that inactivation of Rabaptin-5 and inhibition of vesicle transport lead to fragmentation of endosomes and inhibition of the endocytic pathway during the execution phase of apoptosis. We propose that parallel changes to other membrane transport pathways would give rise to general membrane fragmentation in apoptotic cells. These changes are likely to play an important role in the generation of apoptotic bodies and their recognition by phagocytosing cells.

MeSH Terms
Animals Apoptosis/physiology Biological Transport Cell-Free System Cysteine Endopeptidases/metabolism Cysteine Proteinase Inhibitors/pharmacology Endocytosis Endosomes/physiology Humans Membrane Fusion/drug effects Membrane Proteins/metabolism Ovum Proto-Oncogene Proteins c-bcl-2/metabolism Substrate Specificity Vesicular Transport Proteins Xenopus Xenopus Proteins bcl-X Protein
Chemicals
BCL2L1 protein, Xenopus BCL2L1 protein, human Cysteine Proteinase Inhibitors Membrane Proteins Proto-Oncogene Proteins c-bcl-2 RABEP1 protein, human Vesicular Transport Proteins Xenopus Proteins bcl-X Protein Cysteine Endopeptidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cosulich S C
Division of Biochemistry, School of Biological Sciences, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, UK.
Horiuchi H
Zerial M
Clarke P R
Woodman P G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1997-10-15
Pages
6182-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1326302
Subset
IM
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