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PMID: 9312037 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

The role of U5 snRNP in pre-mRNA splicing.

The EMBO journal ·Vol. 16 ·No. 19 ·1997-10-01 ·Pages 5797-800

Newman AJ

Abstract

The current model for the function of the U5 small nuclear ribonucleoprotein particle (snRNP) in the spliceosome proposes that U5 carries binding sites for the 5' and 3' exons, allowing the spliceosome to 'tether' the 5' exon intermediate produced by the first catalytic step and align it with the 3' exon for the second step. Functional analysis of U5 snRNA in cis-spliceosomes has provided support for this model, and data from nematode and trypanosome splicing systems suggest that U5 or a U5-like snRNA performs a similar role in trans-splicing.

MeSH Terms
Animals Catalysis Exons Humans Introns RNA Precursors/metabolism RNA Splicing Ribonucleoprotein, U5 Small Nuclear/physiology Spliceosomes/physiology
Chemicals
RNA Precursors Ribonucleoprotein, U5 Small Nuclear
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Newman A J
MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, UK. newman@mrc-lmb.com.ac.uk
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24 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.19.5797
Email
newman@mrc-lmb.com.ac.uk
Published
1997-10-01
Pages
5797-800
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170210
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.19.5797 PMC Full Text View journal details
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