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PMID: 9311894 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Influence of membrane anchoring and cytoplasmic domains on the fusogenic activity of vesicular stomatitis virus glycoprotein G.

Journal of virology ·Vol. 71 ·No. 10 ·1997-10-00 ·Pages 7996-8000

Odell D, Wanas E, Yan J, Ghosh HP

Abstract

Chimeric proteins in which the transmembrane anchoring sequence (TM) or both the TM and the cytoplasmic tail (CT) of vesicular stomatitis virus glycoprotein G were replaced with corresponding domains of viral or cellular integral membrane proteins were used to examine the influence of these domains on acidic-pH-induced membrane fusion by G protein. The TM and CT of G were also replaced with the lipid anchor glycosylphosphatidylinositol. Hybrids containing foreign TM or TM and CT sequences were fusogenic at acidic pH but glycosylphosphatidylinositol-anchored G was nonfusogenic at acidic pH. The results suggest that the fusogenic activity of G protein requires membrane anchoring by a hydrophobic peptide sequence and the specific amino acid sequence of the TM has no influence on fusogenic activity.

MeSH Terms
Amino Acid Sequence Animals CD4 Antigens/chemistry,metabolism COS Cells Cell Line Cricetinae Cytoplasm/metabolism Endoplasmic Reticulum/metabolism Glycoproteins/chemistry,metabolism Glycosylphosphatidylinositols/metabolism Golgi Apparatus/metabolism Hydrogen-Ion Concentration Kinetics Membrane Fusion Membrane Glycoproteins Molecular Sequence Data Recombinant Fusion Proteins/biosynthesis,chemistry,metabolism Transfection Vesicular stomatitis Indiana virus/physiology Viral Envelope Proteins/biosynthesis,chemistry,metabolism
Chemicals
CD4 Antigens G protein, vesicular stomatitis virus Glycoproteins Glycosylphosphatidylinositols Membrane Glycoproteins Recombinant Fusion Proteins Viral Envelope Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Odell D
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Wanas E
Yan J
Ghosh H P
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36 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.10.7996-8000.1997
Published
1997-10-00
Pages
7996-8000
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC192161
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.71.10.7996-8000.1997 PMC Full Text View journal details
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