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PMID: 9305642 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Multiple circadian-regulated elements contribute to cycling period gene expression in Drosophila.

The EMBO journal ·Vol. 16 ·No. 16 ·1997-08-15 ·Pages 5006-18

Stanewsky R, Jamison CF, Plautz JD, Kay SA, Hall JC

Abstract

A new regulatory element necessary for the correct temporal expression of the period (per) gene was identified by monitoring real-time per expression in living individual flies carrying two different period-luciferase transgenes. luciferase RNA driven from only the per promoter was not sufficient to replicate the normal pattern of per RNA cycling; however, a per-luc fusion RNA driven from a transgene containing additional per sequences cycled identically to endogenous per. The results indicate the existence of at least two circadian-regulated elements--one within the promoter and one within the transcribed portion of the per gene. Phase and amplitude analysis of both per-luc transgenes revealed that normal per expression requires the regulation of these elements at distinct phases and suggests a mechanism by which biological clocks sustain high-amplitude feedback oscillations.

MeSH Terms
Animals Circadian Rhythm Drosophila/genetics Drosophila Proteins Gene Expression Regulation/genetics Genes, Insect Genes, Reporter Immunohistochemistry Luciferases/genetics,metabolism Luminescent Measurements Nuclear Proteins/biosynthesis,genetics Period Circadian Proteins RNA, Messenger/genetics,metabolism Recombinant Fusion Proteins/metabolism Ribonucleases/metabolism Transgenes
Chemicals
Drosophila Proteins Nuclear Proteins PER protein, Drosophila Period Circadian Proteins RNA, Messenger Recombinant Fusion Proteins Luciferases Ribonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stanewsky R
Department of Biology and National Science Foundation Center for Biological Timing, Brandeis University, Waltham, MA 02254, USA.
Jamison C F
Plautz J D
Kay S A
Hall J C
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.16.5006
Published
1997-08-15
Pages
5006-18
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170135
Subset
IM
Grants
NIGMS NIH HHS · GM-33205 · United States
NIMH NIH HHS · MH-51573 · United States
External Links
PubMed source DOI: 10.1093/emboj/16.16.5006 PMC Full Text View journal details
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