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PMID: 9303321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A small nucleolar RNP protein is required for pseudouridylation of eukaryotic ribosomal RNAs.

The EMBO journal ·Vol. 16 ·No. 15 ·1997-08-01 ·Pages 4770-6

Bousquet-Antonelli C, Henry Y, G'elugne JP, Caizergues-Ferrer M, Kiss T

Abstract

Eukaryotic rRNAs possess numerous post-transcriptionally modified nucleotides. The most abundant modifications, 2'-O-ribose methylation and pseudouridylation, occur in the nucleolus during rRNA processing. The nucleolus contains a large number of small nucleolar RNAs (snoRNAs) most of which can be classified into two distinct families defined by conserved sequence boxes and common associated proteins. The C and D box-containing snoRNAs are associated with fibrillarin, and most of them function as guide RNAs in site-specific ribose methylation of rRNAs. The nucleolar function of the other class of snoRNAs, which share box H and ACA elements and are associated with a glycine- and arginine-rich nucleolar protein, Gar1p, remains elusive. Here we demonstrate that the yeast Saccharomyces cerevisiae Gar1 snoRNP protein plays an essential and specific role in the overall pseudouridylation of yeast rRNAs. These results establish a novel function for Gar1 protein and indicate that the box H/ACA snoRNAs, or at least a subset of these snoRNAs, function in the site-specific pseudouridylation of rRNAs.

MeSH Terms
Alleles Base Sequence DNA Primers/genetics Fungal Proteins/genetics,metabolism Genes, Fungal Nuclear Proteins/genetics,metabolism Polymerase Chain Reaction Pseudouridine/metabolism RNA Processing, Post-Transcriptional RNA, Ribosomal/metabolism RNA, Ribosomal, 18S/metabolism Ribonucleoproteins/genetics,metabolism Ribonucleoproteins, Small Nucleolar Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Temperature
Chemicals
DNA Primers Fungal Proteins Nuclear Proteins RNA, Ribosomal RNA, Ribosomal, 18S Ribonucleoproteins Ribonucleoproteins, Small Nucleolar Saccharomyces cerevisiae Proteins RNA, ribosomal, 25S Pseudouridine GAR1 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bousquet-Antonelli C
Laboratoire de Biologie Mol'eculaire Eucaryote du CNRS, Universit'e Paul Sabatier, Toulouse, France.
Henry Y
G'elugne J P
Caizergues-Ferrer M
Kiss T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.15.4770
Published
1997-08-01
Pages
4770-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170103
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.15.4770 PMC Full Text View journal details
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