Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells.

Nucleic acids research ·Vol. 4 ·No. 10 ·1977-10-00 ·Pages 3581-7

Skup D, Millward S

Abstract

Micrococcal nuclease was used to eliminate endogenous protein synthesis in extracts prepared from L cells. The nuclease can be inhibited subsequently with 2'-deoxythymidine-3', 5'-diphosphate. Nuclease-treated extracts primed with exogenous reovirus mRNA, synthesized full length polypeptides with linear kinetics for almost two hours leading to stimulation of the order of 10(4) times over endogenous background. On the average, between 40 and 50 molecules of polypeptide were synthesized per molecule of mRNA.

MeSH Terms

Animals Aurintricarboxylic Acid/pharmacology Globins/biosynthesis Kinetics L Cells/metabolism Micrococcal Nuclease Protein Biosynthesis/drug effects RNA, Messenger/metabolism Rabbits Reticulocytes/metabolism

Chemicals

RNA, Messenger Aurintricarboxylic Acid Globins Micrococcal Nuclease

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Skup D

Millward S

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.10.3581

Published

1977-10-00

Pages

3581-7

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342674

Subset

External Links

PubMed source DOI: 10.1093/nar/4.10.3581 PMC Full Text View journal details

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Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells.

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