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PMID: 9276953 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The Arabidopsis 14-3-3 multigene family.

Plant physiology ·Vol. 114 ·No. 4 ·1997-08-00 ·Pages 1421-31

Wu K, Rooney MF, Ferl RJ

Abstract

The 14-3-3 proteins are ubiquitous eukaryotic proteins and are encoded by a gene family in many species. We examined the 14-3-3 gene family in Arabidopsis thaliana and found that it contains 10 members. Four new cDNAs, GF14 epsilon, GF14 kappa, GF14 mu, and GF14 nu, and two new genomic clones of GF14 phi and GF14 nu were isolated and characterized. Together with the six previously described 14-3-3 isoforms in Arabidopsis, they constitute a complete family of 10 distinct 14-3-3 proteins of 248 to 268 amino acids. Phylogenetic analysis revealed the presence of two ancient, distinct 14-3-3 gene classes in Arabidopsis and other plants. The epsilon forms diverged early from the other plant isoforms, and plant 14-3-3 genes displayed a different evolutionary course from that of mammals.

MeSH Terms
14-3-3 Proteins Amino Acid Sequence Animals Arabidopsis/genetics Blotting, Southern Evolution, Molecular Genes, Plant Mammals/genetics Molecular Sequence Data Multigene Family Proteins/genetics Sequence Homology, Amino Acid Tyrosine 3-Monooxygenase
Chemicals
14-3-3 Proteins Proteins Tyrosine 3-Monooxygenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wu K
Horticultural Sciences Department, University of Florida, Gainesville 32611, USA.
Rooney M F
Ferl R J
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.114.4.1421
Published
1997-08-00
Pages
1421-31
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC158435
Subset
IM
Grants
NIGMS NIH HHS · R01-GM 40061 · United States
Databases
GENBANK
H36560, H36612, H37251, R29957, R30193, R65375, T21021, T21461, T41737, T41947, T42452, T42492, T42621, T43618, T44005, T45017, T45464, T75753, T88244, T88609, Z18505, Z25944, Z26404, Z29149, Z30731, Z30732, Z32630, Z33784, Z33928, Z47716
External Links
PubMed source DOI: 10.1104/pp.114.4.1421 PMC Full Text View journal details
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