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PMID: 9266833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and functional analysis of new members of STAT induced STAT inhibitor (SSI) family: SSI-2 and SSI-3.

Biochemical and biophysical research communications ·Vol. 237 ·No. 1 ·1997-08-08 ·Pages 79-83

Minamoto S, Ikegame K, Ueno K, Narazaki M, Naka T, Yamamoto H, Matsumoto T, Saito H, Hosoe S, Kishimoto T

Abstract

Upon the corresponding ligand's stimulation, the cytokine receptors activate several signal pathways: JAK-STAT pathway, Ras-MAP kinase pathway and so on. Recently, we demonstrated that one of the STAT3 (signal transducer and activator of transcription-3) target genes could suppress the function of STAT3 and designated as SSI-1(STAT induced STAT inhibitor-1). SSI-1 is thought to play a critical role in negative feedback control of JAK-STAT signaling pathway. In the present study, we identified two novel human genes which products have homologous region in their SH2 domain and its COOH-terminal region to mouse SSI-1. Northern blotting analysis and functional studies demonstrated that SSI-2 and SSI-3 mRNA were also induced by cytokine stimulation and their forced expression in mouse myeloid leukemia cell, M1, suppressed the apoptotic effect of LIF, like SSI-1. We also demonstrated the structure of human SSI-1.

MeSH Terms

Amino Acid Sequence Animals Carrier Proteins/biosynthesis,chemistry,metabolism Cell Division/drug effects Cell Line Cloning, Molecular Cytokines/pharmacology DNA-Binding Proteins Female Growth Inhibitors/pharmacology Humans Interleukin-6 Intracellular Signaling Peptides and Proteins Leukemia Inhibitory Factor Lymphokines/pharmacology Male Mice Molecular Sequence Data Organ Specificity Proteins RNA, Messenger/biosynthesis Recombinant Proteins/biosynthesis,chemistry,metabolism Repressor Proteins Sequence Alignment Sequence Homology, Amino Acid Signal Transduction Suppressor of Cytokine Signaling 1 Protein Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins Trans-Activators Transcription Factors Transcription, Genetic/drug effects Transfection

Chemicals

Carrier Proteins Cytokines DNA-Binding Proteins Growth Inhibitors Interleukin-6 Intracellular Signaling Peptides and Proteins LIF protein, human Leukemia Inhibitory Factor Lif protein, mouse Lymphokines Proteins RNA, Messenger Recombinant Proteins Repressor Proteins SOCS1 protein, human SOCS2 protein, human SOCS3 protein, human Socs1 protein, mouse Socs2 protein, mouse Socs3 protein, mouse Suppressor of Cytokine Signaling 1 Protein Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins Trans-Activators Transcription Factors

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Minamoto S

Department of Medicine III, Osaka University Medical School, Suita, Japan.

Ikegame K

Ueno K

Narazaki M

Naka T

Yamamoto H

Matsumoto T

Saito H

Hosoe S

Kishimoto T

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1006/bbrc.1997.7080

Published

1997-08-08

Pages

79-83

Language

English

Region

United States

NLM ID

0372516

Subset

Databases

GENBANK

AA018910, D31101, W60547

External Links

PubMed source DOI: 10.1006/bbrc.1997.7080 View journal details

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Cloning and functional analysis of new members of STAT induced STAT inhibitor (SSI) family: SSI-2 and SSI-3.

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