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PMID: 9261421 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Priming with live respiratory syncytial virus (RSV) prevents the enhanced pulmonary inflammatory response seen after RSV challenge in BALB/c mice immunized with formalin-inactivated RSV.

Journal of virology ·Vol. 71 ·No. 9 ·1997-09-00 ·Pages 6935-9

Waris ME, Tsou C, Erdman DD, Day DB, Anderson LJ

Abstract

To investigate enhanced disease associated with a formalin-inactivated (FI) respiratory syncytial virus (RSV) vaccine, we studied the pulmonary inflammatory response to RSV in BALB/c mice immunized with live RSV, FI-RSV, or combinations of the two. After RSV challenge, the number of granular cells, the ratio of CD4+/CD8+ lymphocytes, and the level of Th2-like cytokine mRNAs in the bronchoalveolar lavage specimens in mice immunized first with live RSV and then with FI-RSV were lower than that in FI-RSV-immunized mice and close to that in live RSV-immunized mice. These data suggest that prior live RSV infection prevents most of the enhanced inflammatory response seen in FI-RSV-immunized mice and might explain lack of enhanced disease in older FI-RSV-immunized children. A live RSV vaccine might similarly decrease the risk of enhanced disease with non-live RSV vaccines.

MeSH Terms
Animals CD4-CD8 Ratio CD4-Positive T-Lymphocytes/cytology CD8-Positive T-Lymphocytes/cytology Cell Count Chlorocebus aethiops Cytokines/analysis Female Formaldehyde/pharmacology Lung/immunology,virology Mice Mice, Inbred BALB C Pneumonia/etiology,immunology Respiratory Syncytial Viruses/drug effects,immunology Tumor Cells, Cultured Vaccines, Inactivated/adverse effects,immunology Vero Cells Viral Vaccines/adverse effects,immunology
Chemicals
Cytokines Vaccines, Inactivated Viral Vaccines Formaldehyde
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Waris M E
Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Tsou C
Erdman D D
Day D B
Anderson L J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.9.6935-6939.1997
Published
1997-09-00
Pages
6935-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC191977
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.71.9.6935-6939.1997 PMC Full Text View journal details
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