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PMID: 9251186 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of xysA, a gene from Streptomyces halstedii JM8 that encodes a 45-kilodalton modular xylanase, Xys1.

Applied and environmental microbiology ·Vol. 63 ·No. 8 ·1997-08-00 ·Pages 2983-8

Ruiz-Arribas A, Sánchez P, Calvete JJ, Raida M, Fernández-Abalos JM, Santamaría RI

Abstract

The gene xysA from Streptomyces halstedii JM8 encodes a protein of 461 amino acids (Xys1) which is secreted into the culture supernatant as a protein of 45 kDa (Xys1L). Later, this form is proteolytically processed after residue D-362 to produce the protein Xys1S, which conserves the same xylanolytic activity. The cleavage removes a domain of 99 amino acids that shows similarity to bacterial cellulose binding domains and that allows the protein Xys1L to bind to crystalline cellulose (Avicel). Expression of this monocistronic gene is affected by the carbon source present in the culture medium, xylan being the best inducer. By using an anti-Xys1L serum, we have been able to detect xylanases similar in size to Xys1L and Xys1S in most of the different Streptomyces species analyzed, suggesting the ubiquity of these types of xylanases and their processing mechanism.

MeSH Terms
Amino Acid Sequence Bacterial Proteins Blotting, Western Cellulose/metabolism Chromosome Mapping Culture Media/metabolism Gene Expression Molecular Sequence Data Plasmids Recombination, Genetic Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Streptomyces/genetics Transcription, Genetic Xylan Endo-1,3-beta-Xylosidase Xylans/metabolism Xylosidases/genetics,immunology,metabolism
Chemicals
Bacterial Proteins Culture Media Xylans Cellulose Xylosidases Xylan Endo-1,3-beta-Xylosidase Xys1 protein, Streptomyces halstedii
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ruiz-Arribas A
Instituto de Microbiología Bioquímica, Consejo Superior de Investigaciones Científicas (CSIC)/Universidad de Salamanca, Spain.
Sánchez P
Calvete J J
Raida M
Fernández-Abalos J M
Santamaría R I
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.63.8.2983-2988.1997
Published
1997-08-00
Pages
2983-8
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC168597
Subset
IM
Databases
GENBANK
D12647, M64551, M82804, P07986, P11220, P26220, P26514, P26515, P36909, U08894, U41627, Z49894
External Links
PubMed source DOI: 10.1128/aem.63.8.2983-2988.1997 PMC Full Text View journal details
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