Home LiteratureArticle Details
PMID: 9246574 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Neutrophil chemotactic activity and C5a following systemic activation of complement in rats.

Inflammation ·Vol. 21 ·No. 3 ·1997-06-00 ·Pages 325-33

Schmid E, Warner RL, Crouch LD, Friedl HP, Till GO, Hugli TE, Ward PA

Abstract

Using ELISA analysis, rat C5a was stimulated in serum from rats undergoing systemic activation of complement after intravenous infusion of purified cobra venom factor (CVF). Biological (neutrophil chemotactic) activity was also assessed. Serum levels of C5a were directly proportional to the amount of CVF infused. C5a and neutrophil chemotactic activity, peaked by 5 min, then plateaued. In vitro addition of anti-C5a to serum samples of CVF-infused rats totally abolished chemotactic activity, indicating that all biological activity could be ascribed to C5a. Blood neutrophils obtained from CVF-infused animals showed a significant upregulation of CD11b, the increase being reduced (38%) in animals pretreated with anti-C5a. These findings indicate that infusion of CVF into rats produces generation of C5a, all chemotactic activity in serum being related to C5a. The in vivo generation of C5a is, at least inpart, responsible for upregulation of CD11b on blood neutrophils.

MeSH Terms
Animals Antibodies/pharmacology Chemotaxis, Leukocyte/drug effects Complement Activation/drug effects Complement C5a/antagonists & inhibitors,metabolism Elapid Venoms/toxicity In Vitro Techniques Kinetics Lung/drug effects,immunology Lung Injury Macrophage-1 Antigen/metabolism Male Neutrophils/drug effects,immunology Rabbits Rats Up-Regulation
Chemicals
Antibodies Elapid Venoms Macrophage-1 Antigen cobra venom factor Complement C5a
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Schmid E
Department of Pathology, University of Michigan Medical School Ann Arbor 48109, USA.
Warner R L
Crouch L D
Friedl H P
Till G O
Hugli T E
Ward P A
References (16)
16 references, click to expand
  1. The role of complement in the induction and regulation of immune responses.
    Immunology. 1984 Feb;51(2):207-24 PMID: 6363279
  2. Depletion of plasma complement in vivo by a protein of cobra venom: its effect on various immunologic reactions.
    J Immunol. 1970 Jul;105(1):55-69 PMID: 4246566
  3. Immunological, structural and functional relationships between an anti-complementary protein from Crotalus atrox venom, cobra venom factor and human C3.
    Immunology. 1980 Apr;39(4):503-9 PMID: 6769786
  4. Neutrophil-dependent acute lung injury. Requirement for P-selectin (GMP-140).
    J Clin Invest. 1992 Oct;90(4):1600-7 PMID: 1383277
  5. Two anticomplementary factors in cobra venom: hemolysis of guinea pig erythrocytes by one of them.
    J Immunol. 1969 Nov;103(5):944-52 PMID: 4981797
  6. Evidence for role of hydroxyl radical in complement and neutrophil-dependent tissue injury.
    J Clin Invest. 1983 Sep;72 (3):789-801 PMID: 6886005
  7. Primary structure and functional characterization of rat C5a: an anaphylatoxin with unusually high potency.
    Protein Sci. 1994 Aug;3(8):1169-77 PMID: 7987212
  8. Systemic complement activation, lung injury, and products of lipid peroxidation.
    J Clin Invest. 1985 Aug;76(2):517-27 PMID: 4031060
  9. Cobra venom factor: improved method for purification and biochemical characterization.
    J Immunol Methods. 1984 Oct 12;73(1):203-20 PMID: 6491300
  10. Requirement and role of C5a in acute lung inflammatory injury in rats.
    J Clin Invest. 1996 Jul 15;98 (2):503-12 PMID: 8755663
  11. The cobra complement system: I. The alternative pathway of activation.
    Dev Comp Immunol. 1985 Spring;9(2):311-25 PMID: 3894085
  12. Role of beta 2 integrins and ICAM-1 in lung injury following ischemia-reperfusion of rat hind limbs.
    Am J Pathol. 1993 Aug;143(2):464-72 PMID: 8102031
  13. Anaphylatoxins: C3a and C5a.
    Adv Immunol. 1978;26:1-53 PMID: 358800
  14. C5a-induced expression of P-selectin in endothelial cells.
    J Clin Invest. 1994 Sep;94(3):1147-55 PMID: 7521884
  15. Intravascular activation of complement and acute lung injury. Dependency on neutrophils and toxic oxygen metabolites.
    J Clin Invest. 1982 May;69(5):1126-35 PMID: 7068850
  16. Chain structure of cobra venom factor from Naja naja and Naja haje venom.
    Scand J Immunol. 1982 Apr;15(4):357-62 PMID: 7100817
Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
0360-3997
DOI
10.1023/a:1027302017117
Published
1997-06-00
Pages
325-33
Language
English
Region
United States
NLM ID
7600105
Subset
IM
Grants
NIGMS NIH HHS · GM-29507 · United States
NIGMS NIH HHS · GM48477 · United States
NHLBI NIH HHS · HL-07517 · United States
External Links
PubMed source DOI: 10.1023/a:1027302017117 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2