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PMID: 9236196 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Disruption of the Bcl6 gene results in an impaired germinal center formation.

The Journal of experimental medicine ·Vol. 186 ·No. 3 ·1997-08-04 ·Pages 439-48

Fukuda T, Yoshida T, Okada S, Hatano M, Miki T, Ishibashi K, Okabe S, Koseki H, Hirosawa S, Taniguchi M, Miyasaka N, Tokuhisa T

Abstract

The Bcl6 gene has been identified from the chromosomal translocation breakpoint in B cell lymphomas, and its products are expressed highly in germinal center (GC) B cells. To investigate the function of Bcl6 in lymphocytes, we have generated RAG1-deficient mice reconstituted with bone marrow cells from Bcl6-deficient mice (Bcl6(-/-)RM). Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is normal, and Bcl6(-/-)RM produced control levels of primary IgG1 antibodies specific to T cell-dependent antigens. However, GCs were not found in these mice. This defect was mainly due to the abnormalities of B cells. Therefore, Bcl6 is essential for the differentiation of GC B cells.

MeSH Terms
Animals B-Lymphocytes/immunology,metabolism,pathology Cell Differentiation/genetics,immunology DNA-Binding Proteins/genetics Gene Rearrangement, B-Lymphocyte Genes, RAG-1/immunology Germinal Center/immunology,metabolism,pathology Homeodomain Proteins Lymphocyte Activation/genetics Lymphoma, B-Cell/genetics,immunology,pathology Mice Mice, Inbred C57BL Mice, Knockout Spleen/immunology,metabolism,pathology T-Lymphocytes/immunology,metabolism
Chemicals
DNA-Binding Proteins Homeodomain Proteins RAG-1 protein
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Fukuda T
Division of Developmental Genetics, Center for Biomedical Science, Chiba University School of Medicine, Chiba, Japan.
Yoshida T
Okada S
Hatano M
Miki T
Ishibashi K
Okabe S
Koseki H
Hirosawa S
Taniguchi M
Miyasaka N
Tokuhisa T
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.186.3.439
Published
1997-08-04
Pages
439-48
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2199007
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.186.3.439 PMC Full Text View journal details
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