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PMID: 9228668 Published · ppublish English Journal Article

Elevated concentrations of the small stress protein HSP27 in rat renal tumors.

Urological research ·Vol. 25 ·No. 3 ·1997-00-00 ·Pages 173-7

Takashi M, Sakata T, Ohmura M, Kato K

Abstract

The expression of two small stress proteins, alpha B crystallin and the 27-kDa heat shock protein (HSP27), was studied quantitatively and immunohistochemically in normal kidney and renal tumors in rats. Levels of alpha B crystallin in renal cell tumors tended to be higher than in normal kidney (P = 0.07), but with a wide range of values, whereas they were significantly lower in mesenchymal tumors (P < 0.0001). In contrast, HSP27 concentrations in both renal cell (mean +/- SD: 1790 +/- 940 ng/mg protein, n = 15) and mesenchymal (1260 +/- 1080 ng/mg protein, n = 10) tumors were significantly higher than the normal kidney value (142 +/- 30 ng/mg protein, n = 10, P < 0.0001). A positive correlation was found between alpha B crystallin and HSP27 levels limited to the renal cell tumor case (Pearson's correlation coefficient, r = 0.68, P < 0.01). Immunohistochemistry revealed the loops of Henle to be positive for alpha B crystallin, whereas HSP27 staining was positive in glomerular and interstitial vascular walls and epithelial cells of proximal and distal tubules. Positive immunostaining for alpha B crystallin was demonstrated in six of nine renal cell tumors (67%) studied and for HSP27 in all of the nine cases (100%).

MeSH Terms
Animals Carcinoma, Renal Cell/metabolism Crystallins/analysis Heat-Shock Proteins/analysis,metabolism Immunohistochemistry Kidney/chemistry,metabolism Kidney Neoplasms/metabolism Male Mesoderm/metabolism Molecular Chaperones/analysis Rats Rats, Wistar Stress, Physiological/metabolism
Chemicals
Crystallins Heat-Shock Proteins Molecular Chaperones
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Takashi M
Department of Urology, Nagoya University School of Medicine, Japan.
Sakata T
Ohmura M
Kato K
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Article Info
Journal
Urological research
Abbr.
Urol Res
ISSN
0300-5623
DOI
10.1007/BF00941978
Published
1997-00-00
Pages
173-7
Language
English
Region
Germany
NLM ID
0364311
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00941978 View journal details
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