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PMID: 9225049 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A unique segment of the hepatitis B virus group A genotype identified in isolates from South Africa.

The Journal of general virology ·Vol. 78 ( Pt 7) ·1997-07-00 ·Pages 1719-29

Bowyer SM, van Staden L, Kew MC, Sim JG

Abstract

The preS2/S genes of hepatitis B virus isolated from 29 acutely or chronically infected individuals in the Gauteng province of South Africa were sequenced. Phylogenetic analysis of these sequences in comparison with global isolates from the GenBank database showed that 24 sequences clustered with genotypic group A, three with genotypic group D and one each with genotypic groups B and C. Group A isolates had greater identity with groups D (variation of 6.6%) and E (6.8%) than with the Eastern groups B (7.4%) and C (8.1%) and were most different from group F (11.0%). Of the South African group A specimens, 59.1% clustered with two global sequences to form a discrete segment which we have called subgroup A. The amino acid differences that set these isolates apart from the rest of group A tended to cluster in the preS2 region (amino acids 7, 10, 32, 35, 47, 48, 53 and 54), with a few changes occurring in the major surface antigen (amino acid sites 207 and 209). Analysis of isolates showed that there was a 9-fold higher prevalence of the ay determinant in South Africa than previously reported.

MeSH Terms

Amino Acid Sequence Base Sequence DNA, Viral Genetic Variation Genotype Hepatitis B Surface Antigens/genetics Hepatitis B virus/classification,genetics,isolation & purification Humans Molecular Sequence Data Phylogeny Protein Precursors/genetics South Africa

Chemicals

DNA, Viral Hepatitis B Surface Antigens Protein Precursors presurface protein 2, hepatitis B surface antigen

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bowyer S M

National Institute for Virology, University of the Witwatersrand, Sandringham, South Africa.

van Staden L

Kew M C

Sim J G

Article Info

Journal

The Journal of general virology

Abbr.

J Gen Virol

ISSN

0022-1317

DOI

10.1099/0022-1317-78-7-1719

Published

1997-07-00

Pages

1719-29

Language

English

Region

England

NLM ID

0077340

Subset

Databases

GENBANK

U87725, U87726, U87727, U87728, U87729, U87730, U87731, U87732, U87733, U87734, U87735, U87736, U87737, U87738, U87739, U87740, U87741, U87742, U87743, U87744, U87745, U87746, U87747, U87748, U87851, U87852, U88094, U88095, U88096

External Links

PubMed source DOI: 10.1099/0022-1317-78-7-1719 View journal details

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A unique segment of the hepatitis B virus group A genotype identified in isolates from South Africa.

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