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PMID: 9223335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Intracisternal basic fibroblast growth factor enhances functional recovery and up-regulates the expression of a molecular marker of neuronal sprouting following focal cerebral infarction.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 15 ·1997-07-22 ·Pages 8179-84

Kawamata T, Dietrich WD, Schallert T, Gotts JE, Cocke RR, Benowitz LI, Finklestein SP

Abstract

Focal cerebral infarction (stroke) due to unilateral occlusion of the middle cerebral artery in mature rats produces deficits in sensorimotor function of the contralateral limbs that recover partially over time. We found that biweekly intracisternal injection of basic fibroblast growth factor (bFGF; 0.5 microg/injection), a potent neurotrophic polypeptide, markedly enhanced recovery of sensorimotor function of the contralateral limbs during the first month after stroke without apparent adverse side effects. Immunostaining for growth-associated protein 43 (GAP-43), a molecular marker of axonal sprouting, showed a selective increase in GAP-43 immunoreactivity in the intact sensorimotor cortex contralateral to cerebral infarcts following bFGF treatment. These results show that bFGF treatment can enhance functional recovery after stroke, and that the mechanism may include stimulation of neuronal sprouting in the intact brain.

MeSH Terms
Animals Biomarkers Cerebrovascular Disorders/genetics,physiopathology Fibroblast Growth Factor 2/pharmacology GAP-43 Protein Immunohistochemistry Male Membrane Glycoproteins/genetics Nerve Tissue Proteins/genetics Neurons/drug effects,pathology Rats Rats, Sprague-Dawley Up-Regulation
Chemicals
Biomarkers GAP-43 Protein Membrane Glycoproteins Nerve Tissue Proteins Fibroblast Growth Factor 2
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kawamata T
CNS Growth Factor Research Laboratory, Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
Dietrich W D
Schallert T
Gotts J E
Cocke R R
Benowitz L I
Finklestein S P
References (28)
28 references, click to expand
  1. Basic and acidic fibroblast growth factors have trophic effects on neurons from multiple CNS regions.
    J Neurosci. 1988 Jul;8(7):2618-27 PMID: 3249247
  2. Axonal growth-associated proteins.
    Annu Rev Neurosci. 1989;12:127-56 PMID: 2648946
  3. A semiautomated method for measuring brain infarct volume.
    J Cereb Blood Flow Metab. 1990 Mar;10(2):290-3 PMID: 1689322
  4. The growth-associated protein GAP-43 appears in dorsal root ganglion cells and in the dorsal horn of the rat spinal cord following peripheral nerve injury.
    Neuroscience. 1990;34(2):465-78 PMID: 2139720
  5. The pattern of GAP-43 immunostaining changes in the rat hippocampal formation during reactive synaptogenesis.
    Brain Res Mol Brain Res. 1990 Jun;8(1):17-23 PMID: 2166197
  6. Marked protection by moderate hypothermia after experimental traumatic brain injury.
    J Cereb Blood Flow Metab. 1991 Jan;11(1):114-21 PMID: 1983995
  7. Basic fibroblast growth factor prevents thalamic degeneration after cortical infarction.
    J Cereb Blood Flow Metab. 1991 May;11(3):472-8 PMID: 2016355
  8. Concerning the mechanism of recovery in stroke hemiplegia.
    Can J Neurol Sci. 1992 Feb;19(1):57-63 PMID: 1562908
  9. Neocortical localization of tactile/proprioceptive limb placing reactions in the rat.
    Brain Res. 1992 Feb 21;573(1):44-60 PMID: 1576535
  10. Functional reorganization of the brain in recovery from striatocapsular infarction in man.
    Ann Neurol. 1992 May;31(5):463-72 PMID: 1596081
  11. Overgrowth and pruning of dendrites in adult rats recovering from neocortical damage.
    Brain Res. 1992 May 22;581(1):156-60 PMID: 1498666
  12. Pretreatment with intraventricular basic fibroblast growth factor decreases infarct size following focal cerebral ischemia in rats.
    Ann Neurol. 1994 Apr;35(4):451-7 PMID: 8154872
  13. Use-dependent growth of pyramidal neurons after neocortical damage.
    J Neurosci. 1994 Apr;14(4):2140-52 PMID: 8158262
  14. Fibroblast growth factors: activities and significance of non-neurotrophin neurotrophic growth factors.
    Curr Opin Neurobiol. 1994 Feb;4(1):78-86 PMID: 8173328
  15. Endogenous neuroprotection factors and traumatic brain injury: mechanisms of action and implications for therapy.
    J Neurotrauma. 1994 Feb;11(1):3-33 PMID: 8201625
  16. Treatment with basic fibroblastic growth factor following focal cerebral ischemia does not prevent neuronal injury.
    J Neurol Sci. 1995 Jan;128(1):66-70 PMID: 7536816
  17. The effects of a lesion or a peripheral nerve graft on GAP-43 upregulation in the adult rat brain: an in situ hybridization and immunocytochemical study.
    J Neurosci. 1995 May;15(5 Pt 1):3594-611 PMID: 7751932
  18. Overexpression of the neural growth-associated protein GAP-43 induces nerve sprouting in the adult nervous system of transgenic mice.
    Cell. 1995 Oct 20;83(2):269-78 PMID: 7585944
  19. Neocortical neural sprouting, synaptogenesis, and behavioral recovery after neocortical infarction in rats.
    Stroke. 1995 Nov;26(11):2135-44 PMID: 7482662
  20. Delayed treatment with intravenous basic fibroblast growth factor reduces infarct size following permanent focal cerebral ischemia in rats.
    J Cereb Blood Flow Metab. 1995 Nov;15(6):953-9 PMID: 7593356
  21. Selective enhancement of axonal branching of cultured dentate gyrus neurons by neurotrophic factors.
    Neuroscience. 1995 Dec;69(3):763-70 PMID: 8596646
  22. Neural substrates for the effects of rehabilitative training on motor recovery after ischemic infarct.
    Science. 1996 Jun 21;272(5269):1791-4 PMID: 8650578
  23. Intracisternal basic fibroblast growth factor (bFGF) enhances behavioral recovery following focal cerebral infarction in the rat.
    J Cereb Blood Flow Metab. 1996 Jul;16(4):542-7 PMID: 8964792
  24. Intravenous basic fibroblast growth factor decreases brain injury resulting from focal ischemia in cats.
    Stroke. 1997 Mar;28(3):609-15; discussion 615-6 PMID: 9056620
  25. bFGF ameliorates focal ischemic injury by blood flow-independent mechanisms in eNOS mutant mice.
    Am J Physiol. 1997 Mar;272(3 Pt 2):H1401-5 PMID: 9087617
  26. Focal cerebral ischaemia in the rat: 1. Description of technique and early neuropathological consequences following middle cerebral artery occlusion.
    J Cereb Blood Flow Metab. 1981;1(1):53-60 PMID: 7328138
  27. Anatomical distribution of the growth-associated protein GAP-43/B-50 in the adult rat brain.
    J Neurosci. 1988 Jan;8(1):339-52 PMID: 3339416
  28. Transfection of PC12 cells with the human GAP-43 gene: effects on neurite outgrowth and regeneration.
    Brain Res Mol Brain Res. 1990 Jan;7(1):39-44 PMID: 2153893
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.15.8179
Published
1997-07-22
Pages
8179-84
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21577
Subset
IM
Grants
NINDS NIH HHS · F32 NS010828 · United States
NINDS NIH HHS · P01 NS010828 · United States
NINDS NIH HHS · P50 NS010828 · United States
NINDS NIH HHS · NS10828 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.15.8179 PMC Full Text View journal details
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