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PMID: 9223290 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nonexponential kinetics of a single tRNAPhe molecule under physiological conditions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 15 ·1997-07-22 ·Pages 7932-6

Jia Y, Sytnik A, Li L, Vladimirov S, Cooperman BS, Hochstrasser RM

Abstract

The fluorescence decay functions of individual, specifically labeled tRNAPhe molecules exhibit nonexponential character as a result of conformational dynamics occurring during the measurement on a single molecule. tRNAPhe conformational states that interchange much more slowly are evidenced by the distribution of lifetimes observed for many individual molecules. A structural model for the nonexponential decay indicates that the tRNAPhe-probe adduct fluctuates between two states, one of which provides conditions that quench the probe fluorescence.

MeSH Terms
Computer Simulation Fluorescence Kinetics Models, Chemical Nucleic Acid Conformation RNA, Transfer, Phe/chemistry,metabolism
Chemicals
RNA, Transfer, Phe
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jia Y
Chemistry Department, University of Pennsylvania, Philadelphia, PA 19104, USA.
Sytnik A
Li L
Vladimirov S
Cooperman B S
Hochstrasser R M
References (22)
22 references, click to expand
  1. Single molecule emission characteristics in near-field microscopy.
    Phys Rev Lett. 1995 Dec 25;75(26):4772-4775 PMID: 10059993
  2. New fluorescent dyes in the red region for biodiagnostics.
    J Fluoresc. 1995 Sep;5(3):247-61 PMID: 24226792
  3. Alterations of single molecule fluorescence lifetimes in near-field optical microscopy.
    Science. 1994 Jul 15;265(5170):364-7 PMID: 17838037
  4. [Preparative isolation of individual tRNA Phe by high performance liquid reversed-phase chromatography].
    Bioorg Khim. 1988 Jan;14(1):27-30 PMID: 2454622
  5. Donor-acceptor distance distributions in a double-labeled fluorescent oligonucleotide both as a single strand and in duplexes.
    Biochemistry. 1995 Jan 10;34(1):293-300 PMID: 7819210
  6. Distance moved by transfer RNA during translocation from the A site to the P site on the ribosome.
    J Mol Biol. 1982 Mar 25;156(1):113-40 PMID: 6178833
  7. Single molecules observed by near-field scanning optical microscopy.
    Science. 1993 Nov 26;262(5138):1422-5 PMID: 17736823
  8. Real time imaging of single fluorophores on moving actin with an epifluorescence microscope.
    Biophys J. 1995 Aug;69(2):323-8 PMID: 8527645
  9. Sorting single molecules: application to diagnostics and evolutionary biotechnology.
    Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5740-7 PMID: 7517036
  10. Probing individual molecules with confocal fluorescence microscopy.
    Science. 1994 Nov 11;266(5187):1018-21 PMID: 7973650
  11. Further refinement of the structure of yeast tRNAPhe.
    J Mol Biol. 1978 Sep 25;124(3):523-34 PMID: 361973
  12. Direct observation of single kinesin molecules moving along microtubules.
    Nature. 1996 Apr 4;380(6573):451-3 PMID: 8602245
  13. Probing single molecule dynamics.
    Science. 1994 Jul 15;265(5170):361-4 PMID: 17838036
  14. Imaging of single molecule diffusion.
    Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):2926-9 PMID: 8610144
  15. Conformational transitions monitored for single molecules in solution.
    Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6710-5 PMID: 8692883
  16. Intermittency of single molecule reaction dynamics in fluctuating environments.
    Phys Rev Lett. 1995 May 22;74(21):4317-4320 PMID: 10058470
  17. On the analysis of fluorescence decay kinetics by the method of least-squares.
    Anal Biochem. 1974 Jun;59(2):583-98 PMID: 4838786
  18. Three-dimensional imaging of single molecules solvated in pores of poly(acrylamide) gels.
    Science. 1996 Nov 8;274(5289):966-9 PMID: 8875935
  19. Single Molecule Dynamics Studied by Polarization Modulation.
    Phys Rev Lett. 1996 Nov 4;77(19):3979-3982 PMID: 10062357
  20. Single molecule fluorescence burst detection of DNA fragments separated by capillary electrophoresis.
    Anal Chem. 1995 Sep 15;67(18):3253-60 PMID: 11407410
  21. Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor.
    Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6264-8 PMID: 8692803
  22. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution.
    Nature. 1995 Apr 6;374(6522):555-9 PMID: 7700383
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.15.7932
Published
1997-07-22
Pages
7932-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC21532
Subset
IM
Grants
NCRR NIH HHS · RR01348-14 · United States
NIGMS NIH HHS · GM53416 · United States
NIGMS NIH HHS · R37 GM012592 · United States
NIGMS NIH HHS · GM12592 · United States
NCRR NIH HHS · P41 RR001348 · United States
NIGMS NIH HHS · R01 GM012592 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.15.7932 PMC Full Text View journal details
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