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PMID: 9199172 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human Bcl-2 reverses survival defects in yeast lacking superoxide dismutase and delays death of wild-type yeast.

The Journal of cell biology ·Vol. 137 ·No. 7 ·1997-06-30 ·Pages 1581-8

Longo VD, Ellerby LM, Bredesen DE, Valentine JS, Gralla EB

Abstract

We expressed the human anti-apoptotic protein, Bcl-2, in Saccharomyces cerevisiae to investigate its effects on antioxidant protection and stationary phase survival. Yeast lacking copper-zinc superoxide dismutase (sod1Delta) show a profound defect in entry into and survival during stationary phase even under conditions optimal for survival of wild-type strains (incubation in water after stationary phase is reached). Expression of Bcl-2 in the sod1Delta strain caused a large improvement in viability at entry into stationary phase, as well as increased resistance to 100% oxygen and increased catalase activity. In addition, Bcl-2 expression reduced mutation frequency in both wild-type and sod1Delta strains. In another set of experiments, wild-type yeast incubated in expired minimal medium instead of water lost viability quickly; expression of Bcl-2 significantly delayed this stationary phase death. Our results demonstrate that Bcl-2 has activities in yeast that are similar to activities it is known to possess in mammalian cells: (a) stimulation of antioxidant protection and (b) delay of processes leading to cell death.

MeSH Terms
Gene Expression Regulation, Fungal Gene Transfer Techniques Humans Mutation Oxidative Stress Proto-Oncogene Proteins c-bcl-2/genetics Saccharomyces cerevisiae/genetics,growth & development Superoxide Dismutase/genetics
Chemicals
Proto-Oncogene Proteins c-bcl-2 Superoxide Dismutase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Longo V D
Department of Chemistry and Biochemistry, University of California at Los Angeles, Los Angeles, California 90095-1569, USA.
Ellerby L M
Bredesen D E
Valentine J S
Gralla E B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.137.7.1581
Published
1997-06-30
Pages
1581-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2137818
Subset
IM
Grants
NINDS NIH HHS · NS25554 · United States
NIDDK NIH HHS · DK46828 · United States
NIA NIH HHS · AG12282 · United States
External Links
PubMed source DOI: 10.1083/jcb.137.7.1581 PMC Full Text View journal details
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