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PMID: 9197419 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and genetic mapping of wheat telomere-associated sequences.

Molecular & general genetics : MGG ·Vol. 254 ·No. 5 ·1997-05-20 ·Pages 584-91

Mao L, Devos KM, Zhu L, Gale MD

Abstract

Wheat telomere-associated sequences (TASs) were cloned using a Vectorette approach and sequenced. Reverse primers specific to the TASs were combined with labelled degenerate telomere primers in PCR reactions containing total genomic DNA as template. Amplification products were separated on sequencing gels. In total, seventeen primer combinations provided 47 polymorphic fragments. Nine of these mapped beyond the most distal RFLP markers and defined the ends of seven chromosome arms. Seven of the nine terminal fragments were derived from a 118-bp tandem repeat, indicating that subtelomeric tandem repeat sequences provide an efficient means to target chromosome ends. A telomere cloning strategy and the terminal and interstitial location of TASs are discussed.

MeSH Terms
Base Sequence Chromosome Mapping Cloning, Molecular Genetic Markers Molecular Sequence Data Polymerase Chain Reaction/methods Repetitive Sequences, Nucleic Acid/genetics Sequence Analysis, DNA Telomere/genetics Triticum/genetics
Chemicals
Genetic Markers
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mao L
John Innes Centre, Norwich Research Park, Colney, UK.
Devos K M
Zhu L
Gale M D
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/s004380050455
Published
1997-05-20
Pages
584-91
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
Z75559, Z75560, Z75561, Z75562, Z75563, Z75564, Z75565, Z75566, Z75567, Z75568, Z75569, Z75570, Z75571, Z75572, Z75573, Z75574, Z75575, Z75576
External Links
PubMed source DOI: 10.1007/s004380050455
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