Molecular species analysis of phospholipids.

The elucidation of phospholipid molecular species composition provides detailed structural information concerning various lipids and thus offers descriptions of crucial determinants of membrane physical and biological properties. Various methods differing in labor intensity, mode of separation and detection, type of calibration, as well as other factors, have been published. Thus precision and accuracy are expected to vary considerably between methods. Qualitative and quantitative aspects of different procedures for molecular species analysis of individual phospholipid classes are discussed in this review. Special emphasis has been given to the characterization of biological tissue samples.