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PMID: 9184237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The integration of nitrogen and carbon catabolite repression in Aspergillus nidulans requires the GATA factor AreA and an additional positive-acting element, ADA.

The EMBO journal ·Vol. 16 ·No. 10 ·1997-05-15 ·Pages 2937-44

Gonzalez R, Gavrias V, Gomez D, Scazzocchio C, Cubero B

Abstract

The expression of the structural genes of the proline utilization cluster of Aspergillus nidulans is repressed efficiently only when both repressing carbon and nitrogen sources are present. Two hypotheses can account for this fact. One is a direct or indirect competition mechanism between the positive-acting AreA GATA factor, mediating nitrogen metabolite repression, and the negative-acting CreA protein, mediating carbon catabolite repression. The second is to propose that CreA prevents the binding or activity of another, as yet unidentified, positive-acting factor, here called ADA. We show the second possibility to be the correct one, and we localize the new positive cis-acting element within 290 bp of the prnD-prnB divergent promoter.

MeSH Terms
Aspergillus nidulans/genetics Binding, Competitive Carbon/metabolism Chromosome Mapping Cloning, Molecular Fungal Proteins/metabolism Gene Expression Regulation, Fungal Models, Genetic Molecular Sequence Data Multigene Family Nitrogen/metabolism Proline/biosynthesis Promoter Regions, Genetic Protein Binding Repressor Proteins/metabolism Sequence Analysis, DNA Sequence Deletion Transcription Factors/metabolism Transformation, Genetic
Chemicals
AreA protein, Aspergillus nidulans Fungal Proteins Repressor Proteins Transcription Factors CreA protein, Aspergillus nidulans Carbon Proline Nitrogen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gonzalez R
Institut de Génétique et Microbiologie, Unité de Recherche Associée au CNRS D2225, Université Paris-Sud, Orsay, France. rgonzalez@igmors.u-psud.fr
Gavrias V
Gomez D
Scazzocchio C
Cubero B
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31 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.10.2937
Email
rgonzalez@igmors.u-psud.fr
Published
1997-05-15
Pages
2937-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1169901
Subset
IM
Databases
GENBANK
U74465
External Links
PubMed source DOI: 10.1093/emboj/16.10.2937 PMC Full Text View journal details
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