Home LiteratureArticle Details

PMID: 91687 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2.

Journal of virology ·Vol. 32 ·No. 2 ·1979-11-00 ·Pages 676-8

Zweig M, Heilman CJ, Rabin H, Hopkins RF, Neubauer RH, Hampar B

Abstract

We prepared mouse hybrid cell lines which produced antibodies against herpes simplex virus type 1 and 2 nucleocapsids. Cell lines 1D4 and 3E1, respectively, secreted immunoglobulin G1 herpes simplex virus type 1 and immunoglobulin G1 herpes simplex virus type 2 antibodies which immunoprecipitated proteins designated p40 and p45 from homologous nucleocapsid preparations but precipitated no proteins from heterologous preparations. In contrast, guinea pig antisera prepared against either herpes simplex virus type 1 or 2 p40 precipitated p40 and p45 from both homologous and heterologous preparations. These findings suggest that p40 and p45 possess similar antigenic determinants and that the monoclonal antibodies that were tested reacted preferentially with the homologous determinants.

MeSH Terms

Antibodies, Viral/biosynthesis Antigens, Viral/analysis Capsid/immunology Cell Line Epitopes Hybrid Cells Immunoglobulin G/biosynthesis Simplexvirus/immunology Viral Proteins/immunology

Chemicals

Antibodies, Viral Antigens, Viral Epitopes Immunoglobulin G Viral Proteins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Zweig M

Heilman C J

Rabin H

Hopkins R F

Neubauer R H

Hampar B

References (12)

12 references, click to expand

Continuous cultures of fused cells secreting antibody of predefined specificity.
Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses.
Virology. 1979 Feb;93(1):111-26 PMID: 219593
Identification of disulfide-linked protein complexes in the nucleocapsids of herpes simplex virus type 2.
Virology. 1979 Apr 30;94(2):442-50 PMID: 222065
Mouse hybrid cell lines produce antibodies to herpes simplex virus type 1.
J Gen Virol. 1979 Jul;44(1):81-7 PMID: 228002
Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells.
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2985-8 PMID: 268647
Proteins specified by herpes simplex virus. Staining and radiolabeling properties of B capsid and virion proteins in polyacrylamide gels.
J Virol. 1974 Jan;13(1):155-65 PMID: 4129836
Proteins specified by herpes simplex virus. 8. Characterization and composition of multiple capsid forms of subtypes 1 and 2.
J Virol. 1972 Nov;10(5):1044-52 PMID: 4344252
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Somatic cell hybrids producing antibodies specific for the tumor antigen of simian virus 40.
Proc Natl Acad Sci U S A. 1978 May;75(5):2320-3 PMID: 79181
Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.
Eur J Immunol. 1976 Jul;6(7):511-9 PMID: 825377
Isolation of a nucleocapsid polypeptide of herpes simplex virus types 1 and 2 possessing immunologically type-specific and cross-reactive determinants.
J Virol. 1979 Jan;29(1):34-42 PMID: 85720

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.32.2.676-678.1979

Published

1979-11-00

Pages

676-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC353600

Subset

External Links

PubMed source DOI: 10.1128/JVI.32.2.676-678.1979 PMC Full Text View journal details

Analysis Services

Production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2.

Contact

WeChat Official Account