Home LiteratureArticle Details
PMID: 9168453 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Metal ion stimulation of phospholipase D-like activity of isolated rat intestinal mitochondria.

Lipids ·Vol. 32 ·No. 5 ·1997-05-00 ·Pages 471-9

Madesh M, Balasubramanian KA

Abstract

Presence of phospholipase D-like (PLD) activity in the intestinal mitochondria was identified using endogenous phospholipids as substrate. The enzyme had a pH optimum of 6.5, did not show trans-phosphatidylation activity in the presence of ethanol or butanol, and the product formed was phosphatidic acid (PA). This was confirmed by separation of reaction products by high-performance liquid chromatography and analysis of composition of the PA formed which gave phosphate/fatty acid ratio of 1:2 PLD-like activity was further confirmed by the formation of ethanolamine and choline as products of enzyme action. This activity was stimulated by various metal ions; when stimulated by Mg2+ and Ba2+, it hydrolyzed both phosphatidylcholine and phosphatidylethanolamine, and when stimulated by Ca2+, it preferentially hydrolyzed phosphatidylethanolamine. There was no requirement for sodium oleate for the PLD-like activity in mitochondria. These results suggest that intestinal mitochondria have an active PLD-like enzyme which differs in certain properties from phospholipase D from other tissues.

MeSH Terms
Animals Cations, Divalent/pharmacology Choline/metabolism Chromatography, High Pressure Liquid Chromatography, Thin Layer Ethanolamine Ethanolamines/metabolism Fluorometry Hydrogen-Ion Concentration Intestines/drug effects,enzymology Metals/pharmacology Mitochondria/drug effects,enzymology Phosphatidic Acids/chemistry,metabolism Phospholipase D/metabolism Phospholipids/metabolism Rats
Chemicals
Cations, Divalent Ethanolamines Metals Phosphatidic Acids Phospholipids Ethanolamine Phospholipase D Choline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Madesh M
Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College & Hospital, Vellore, India.
Balasubramanian K A
References (40)
40 references, click to expand
  1. Phospholipase D activity of human amnion cells stimulated with phorbol ester and bradykinin.
    Biochim Biophys Acta. 1993 Jun 12;1168(2):213-9 PMID: 8504157
  2. Phospholipase D in cell signalling and its relationship to phospholipase C.
    Life Sci. 1991;48(9):851-66 PMID: 1997787
  3. [Properties of phospholipase D from rat liver mitochondria].
    Biokhimiia. 1989 Jul;54(7):1066-74 PMID: 2804163
  4. Protein kinase C alpha mediates phospholipase D activation by nucleotides and phorbol ester in Madin-Darby canine kidney cells. Stimulation of phospholipase D is independent of activation of polyphosphoinositide-specific phospholipase C and phospholipase A2.
    J Biol Chem. 1994 Apr 8;269(14 ):10511-6 PMID: 8144636
  5. A new method for the direct determination of serum cholesterol.
    J Lab Clin Med. 1953 Mar;41(3):486-92 PMID: 13035283
  6. Characterization of phospholipase D in a cell-free system of cultured cells derived from rat frontal cortex.
    Brain Res. 1992 Nov 6;595(1):12-6 PMID: 1467947
  7. Quantification of lysophosphatidylethanolamine in the nanomole range.
    J Lipid Res. 1981 Jul;22(5):882-6 PMID: 7288296
  8. Mitochondrial calcium transport: physiological and pathological relevance.
    Am J Physiol. 1994 Aug;267(2 Pt 1):C313-39 PMID: 8074170
  9. Phosphorus assay in column chromatography.
    J Biol Chem. 1959 Mar;234(3):466-8 PMID: 13641241
  10. Phospholipids as ionophores.
    J Biol Chem. 1976 Mar 10;251(5):1326-32 PMID: 1254569
  11. Studies on nonesterified fatty acids in rat intestinal cell membranes.
    Indian J Biochem Biophys. 1993 Oct;30(5):285-8 PMID: 8144173
  12. Identification and partial characterization of phospholipase D in the human amniotic membrane.
    Biochem Biophys Res Commun. 1993 Mar 31;191(3):1270-7 PMID: 8466504
  13. Oxidative stress in mitochondria: its relationship to cellular Ca2+ homeostasis, cell death, proliferation, and differentiation.
    Chem Biol Interact. 1991;77(1):1-23 PMID: 1983962
  14. Synergistic activation of phospholipase D by protein kinase C- and G-protein-mediated pathways in streptolysin O-permeabilized HL60 cells.
    Biochem J. 1992 Jun 1;284 ( Pt 2):531-8 PMID: 1599436
  15. Comparison of phospholipid and fatty acid composition of human erythrocytes and platelets.
    Br J Haematol. 1969 Oct;17(4):359-71 PMID: 5346409
  16. Receptor- and phorbol ester-mediated phospholipase D activation in rat parotid involves two different pathways.
    Am J Physiol. 1994 Mar;266(3 Pt 1):C692-9 PMID: 7909406
  17. Hydrogen peroxide regulates phospholipase D-mediated hydrolysis of phosphatidylethanolamine and phosphatidylcholine by different mechanisms in NIH 3T3 fibroblasts.
    Arch Biochem Biophys. 1994 Jun;311(2):430-6 PMID: 8203906
  18. Calcium-dependent mitochondrial oxidative damage promoted by 5-aminolevulinic acid.
    Biochim Biophys Acta. 1992 Dec 10;1180(2):201-6 PMID: 1463771
  19. Properties of microsomal and soluble diacylglycerol kinase in rat liver.
    Eur J Biochem. 1978 Apr;85(1):225-32 PMID: 205418
  20. A neutral phospholipase D activity from rat brain synaptic plasma membranes. Identification and partial characterization.
    J Biol Chem. 1990 Oct 15;265(29):17512-9 PMID: 2211645
  21. Partial purification and properties of a rat brain phospholipase.
    J Biol Chem. 1979 Oct 10;254(19):9761-5 PMID: 489566
  22. A simplified spectrophotometric determination of ester groups in lipids.
    Biochim Biophys Acta. 1959 Jul;34:244-5 PMID: 13832326
  23. Pro-oxidants and mitochondrial Ca2+: their relationship to apoptosis and oncogenesis.
    FEBS Lett. 1993 Jun 28;325(1-2):104-7 PMID: 8513880
  24. CTP: phosphocholine cytidylyltransferase from rat liver.
    Methods Enzymol. 1981;71 Pt C:576-81 PMID: 6268934
  25. Phosphatidylethanol formation via transphosphatidylation by rat brain synaptosomal phospholipase D.
    J Neurochem. 1987 May;48(5):1597-603 PMID: 3559569
  26. Microsomal phospholipase D of rat brain and lung tissues.
    Biochem Biophys Res Commun. 1980 Sep 30;96(2):742-7 PMID: 7426012
  27. Phospholipase D-mediated hydrolysis of phosphatidylcholine: role in cell signalling.
    J Lipid Mediat. 1993 Nov;8(3):177-82 PMID: 8268464
  28. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  29. Phospholipase D activation in fibroblast membranes by the alpha and beta isoforms of protein kinase C.
    FEBS Lett. 1994 Apr 4;342(2):149-53 PMID: 8143868
  30. Membrane-associated phospholipase D activity in rat sciatic nerve.
    J Neurochem. 1991 Oct;57(4):1429-36 PMID: 1895113
  31. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  32. Effect of exposure of various oxidants on rat liver and intestinal microsomes--a comparative study.
    Chem Biol Interact. 1991;80(2):135-44 PMID: 1657421
  33. Lipopolysaccharide-mediated signal transduction through phospholipase D activation in monocytic cell lines.
    Biochim Biophys Acta. 1994 Jun 23;1213(1):14-20 PMID: 8011674
  34. Activation of endothelial cell phospholipase D by hydrogen peroxide and fatty acid hydroperoxide.
    J Biol Chem. 1993 Jan 15;268(2):930-7 PMID: 8419372
  35. The role of cytosolic Ca2+, protein kinase C, and protein kinase A in hormonal stimulation of phospholipase D in rat hepatocytes.
    J Biol Chem. 1994 Jan 14;269(2):849-59 PMID: 8288638
  36. Quantitative analysis of phospholipids by thin-layer chromatography.
    Biochem J. 1964 Feb;90(2):374-8 PMID: 4284220
  37. Rapid activation of phosphatidate phosphohydrolase in mesangial cells by lipid A.
    Biochemistry. 1991 Jun 25;30(25):6195-203 PMID: 1905569
  38. Activation of rat liver plasma-membrane diacylglycerol kinase by vasopressin and phenylephrine.
    Biochem J. 1988 Nov 1;255 (3):923-8 PMID: 2850802
  39. Phospholipase D activity of isolated rat brain plasma membranes.
    FEBS Lett. 1994 Jan 17;337(3):251-4 PMID: 8293809
  40. Is phosphatidic acid a calcium ionophore under neurohumoral control?
    Nature. 1980 Mar 27;284(5754):345-7 PMID: 6244502
Article Info
Journal
Lipids
Abbr.
Lipids
ISSN
0024-4201
DOI
10.1007/s11745-997-0061-9
Published
1997-05-00
Pages
471-9
Language
English
Region
United States
NLM ID
0060450
Subset
IM
External Links
PubMed source DOI: 10.1007/s11745-997-0061-9 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2