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PMID: 9154830 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential requirements for DNA replication in the activation of mitotic checkpoints in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 17 ·No. 6 ·1997-06-00 ·Pages 3315-22

Tavormina PA, Wang Y, Burke DJ

Abstract

Checkpoints prevent inaccurate chromosome segregation by inhibiting cell division when errors in mitotic processes are encountered. We used a temperature-sensitive mutation, dbf4, to examine the requirement for DNA replication in establishing mitotic checkpoint arrest. We used gamma-irradiation to induce DNA damage and hydroxyurea to limit deoxyribonucleotides in cells deprived of DBF4 function to investigate the requirement for DNA replication in DNA-responsive checkpoints. In the absence of DNA replication, mitosis was not inhibited by these treatments, which normally activate the DNA damage and DNA replication checkpoints. Our results support a model that indicates that the assembly of replication structures is critical for cells to respond to defects in DNA metabolism. We show that activating the spindle checkpoint with nocodazole does not require prior progression through S phase but does require a stable kinetochore.

MeSH Terms
Antineoplastic Agents/pharmacology Cell Cycle Proteins Cell Division Chromatids/metabolism DNA/metabolism DNA Damage DNA Replication DNA, Fungal/metabolism Fungal Proteins/metabolism Kinetochores/metabolism Mitosis/physiology Nocodazole/pharmacology Nuclear Proteins/metabolism S Phase Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Spindle Apparatus/metabolism
Chemicals
Antineoplastic Agents CTF13 protein, S cerevisiae Cell Cycle Proteins DNA, Fungal Dbf4 protein, S cerevisiae Fungal Proteins Nuclear Proteins Saccharomyces cerevisiae Proteins DNA Nocodazole
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tavormina P A
Department of Biology, University of Virginia, Charlottesville 22903, USA.
Wang Y
Burke D J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.6.3315
Published
1997-06-00
Pages
3315-22
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232184
Subset
IM
Grants
NIGMS NIH HHS · GM 40334 · United States
External Links
PubMed source DOI: 10.1128/MCB.17.6.3315 PMC Full Text View journal details
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