Home LiteratureArticle Details
PMID: 9138572 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Effect of hydrostatic pressure on water penetration and rotational dynamics in phospholipid-cholesterol bilayers.

Biophysical journal ·Vol. 72 ·No. 3 ·1997-03-00 ·Pages 1264-77

Bernsdorff C, Wolf A, Winter R, Gratton E

Abstract

The effect of high hydrostatic pressure on the lipid bilayer hydration, the mean order parameter, and rotational dynamics of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) cholesterol vesicles has been studied by time-resolved fluorescence spectroscopy up to 1500 bar. Whereas the degree of hydration in the lipid headgroup and interfacial region was assessed from fluorescence lifetime data using the probe 1-(4-trimethylammonium-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH), the corresponding information in the upper acyl chain region was estimated from its effect on the fluorescence lifetime of and 3-(diphenylhexatrienyl)propyl-trimethylammonium (TMAP-DPH). The lifetime data indicate a greater level of interfacial hydration for DPPC bilayers than for POPC bilayers, but there is no marked difference in interchain hydration of the two bilayer systems. The addition of cholesterol at levels from 30 to 50 mol% to DPPC has a greater effect on the increase of hydrophobicity in the interfacial region of the bilayer than the application of hydrostatic pressure of several hundred to 1000 bar. Although the same trend is observed in the corresponding system, POPC/30 mol% cholesterol, the observed effects are markedly less pronounced. Whereas the rotational correlation times of the fluorophores decrease in passing the pressure-induced liquid-crystalline to gel phase transition of DPPC, the wobbling diffusion coefficient remains essentially unchanged. The wobbling diffusion constant of the two fluorophores changes markedly upon incorporation of 30 mol% cholesterol, and increases at higher pressures, also in the case of POPC/30 mol% cholesterol. The observed effects are discussed in terms of changes in the rotational characteristics of the fluorophores and the phase-state of the lipid mixture. The results demonstrate the ability of cholesterol to adjust the structural and dynamic properties of membranes composed of different phospholipid components, and to efficiently regulate the motional freedom and hydrophobicity of membranes, so that they can withstand even drastic changes in environmental conditions, such as high external hydrostatic pressure.

MeSH Terms
1,2-Dipalmitoylphosphatidylcholine/chemistry Cholesterol/chemistry Diphenylhexatriene/analogs & derivatives Fluorescent Dyes Kinetics Lipid Bilayers/chemistry Molecular Conformation Phosphatidylcholines/chemistry Rotation Spectrometry, Fluorescence Structure-Activity Relationship Time Factors
Chemicals
Fluorescent Dyes Lipid Bilayers Phosphatidylcholines Diphenylhexatriene 1,2-Dipalmitoylphosphatidylcholine 1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene Cholesterol 1-palmitoyl-2-oleoylphosphatidylcholine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bernsdorff C
University of Dortmund, Department of Chemistry, Germany.
Wolf A
Winter R
Gratton E
References (68)
68 references, click to expand
  1. Differential scanning calorimetric study of the effect of sterol side chain length and structure on dipalmitoylphosphatidylcholine thermotropic phase behavior.
    Biophys J. 1995 Jul;69(1):169-76 PMID: 7669894
  2. Lateral organization of liquid-crystalline cholesterol-dimyristoylphosphatidylcholine bilayers. Evidence for domains with hexagonal and centered rectangular cholesterol superlattices.
    Biochemistry. 1995 Sep 12;34(36):11568-81 PMID: 7547888
  3. Hydrostatic pressure-induced conformational changes in phosphatidylcholine headgroups: a 2H NMR study.
    Biophys J. 1995 Aug;69(2):518-23 PMID: 8527666
  4. Membrane organization at low cholesterol concentrations: a study using 7-nitrobenz-2-oxa-1,3-diazol-4-yl-labeled cholesterol.
    Biochemistry. 1996 Jan 30;35(4):1311-22 PMID: 8573588
  5. Rotational diffusion of a steroid molecule in phosphatidylcholine-cholesterol membranes: fluid-phase microimmiscibility in unsaturated phosphatidylcholine-cholesterol membranes.
    Biochemistry. 1990 May 1;29(17):4059-69 PMID: 2163271
  6. Phosphatidic acid affects structural organization of phosphatidylcholine liposomes. A study of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammonium-phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) fluorescence decay using distributional analysis.
    Chem Phys Lipids. 1990 Jul;55(1):29-39 PMID: 2208443
  7. The effect of high external pressure on DPPC-cholesterol multilamellar vesicles: a pressure-tuning Fourier transform infrared spectroscopy study.
    Biochim Biophys Acta. 1996 Feb 21;1279(1):5-16 PMID: 8624361
  8. Fluorescence lifetime distributions of diphenylhexatriene-labeled phosphatidylcholine as a tool for the study of phospholipid-cholesterol interactions.
    Biophys J. 1989 Dec;56(6):1245-53 PMID: 2611334
  9. Phase equilibria of cholesterol/dipalmitoylphosphatidylcholine mixtures: 2H nuclear magnetic resonance and differential scanning calorimetry.
    Biochemistry. 1990 Jan 16;29(2):451-64 PMID: 2302384
  10. Relationships between lipid membrane area, hydrophobic thickness, and acyl-chain orientational order. The effects of cholesterol.
    Biophys J. 1990 Mar;57(3):405-12 PMID: 2306491
  11. Modulation of phospholipid acyl chain order by cholesterol. A solid-state 2H nuclear magnetic resonance study.
    Biochemistry. 1990 Nov 27;29(47):10676-84 PMID: 2271675
  12. A photophysical model for diphenylhexatriene fluorescence decay in solvents and in phospholipid vesicles.
    Biophys J. 1991 Feb;59(2):466-75 PMID: 2009361
  13. Compression of lipid membranes as observed at varying membrane positions.
    Biophys J. 1991 Aug;60(2):334-40 PMID: 1912276
  14. Analysis of cell membrane micro-heterogeneity using the fluorescence lifetime of DPH-type fluorophores.
    Biochim Biophys Acta. 1992 Mar 2;1104(2):273-82 PMID: 1547264
  15. High-pressure 31P NMR study of dipalmitoylphosphatidylcholine bilayers.
    Biochemistry. 1992 Jul 21;31(28):6383-90 PMID: 1633150
  16. Polarity of lipid bilayers. A fluorescence investigation.
    Biochemistry. 1992 Aug 25;31(33):7672-82 PMID: 1510953
  17. Hydration at the membrane protein-lipid interface.
    Biophys J. 1992 Oct;63(4):897-902 PMID: 1384744
  18. E/M dips. Evidence for lipids regularly distributed into hexagonal super-lattices in pyrene-PC/DMPC binary mixtures at specific concentrations.
    Biophys J. 1992 Oct;63(4):903-10 PMID: 1420934
  19. A 13C and 2H nuclear magnetic resonance study of phosphatidylcholine/cholesterol interactions: characterization of liquid-gel phases.
    Biochemistry. 1993 Dec 7;32(48):13277-87 PMID: 8241184
  20. Influence of the local anesthetic tetracaine on the phase behavior and the thermodynamic properties of phospholipid bilayers.
    Biophys J. 1993 Nov;65(5):2041-6 PMID: 8298033
  21. Pressure effects on the physical properties of lipid bilayers detected by trans-parinaric acid fluorescence decay.
    Biophys J. 1993 Nov;65(5):2248-60 PMID: 8298048
  22. Fluorescence dynamics of diphenyl-1,3,5-hexatriene-labeled phospholipids in bilayer membranes.
    Biophys Chem. 1994 Jan;48(3):337-51 PMID: 8110960
  23. Influence of the length of the spacer on the partitioning properties of amphiphilic fluorescent membrane probes.
    Chem Phys Lipids. 1993 Nov;66(1-2):135-42 PMID: 8118914
  24. Pressure-induced elevation of phase transition temperature in dipalmitoylphosphatidylcholine bilayers. An electron spin resonance measurement of the enthalpy of phase transition.
    Biochim Biophys Acta. 1974 Dec 24;373(3):436-43 PMID: 4373076
  25. The function of sterols in membranes.
    Biochim Biophys Acta. 1976 Oct 26;457(2):109-32 PMID: 184844
  26. Deuterium magnetic resonance: theory and application to lipid membranes.
    Q Rev Biophys. 1977 Aug;10(3):353-418 PMID: 335428
  27. High-sensitivity scanning calorimetric study of mixtures of cholesterol with dimyristoyl- and dipalmitoylphosphatidylcholines.
    Biochemistry. 1978 Jun 13;17(12):2464-8 PMID: 581060
  28. Fluidity parameters of lipid regions determined by fluorescence polarization.
    Biochim Biophys Acta. 1978 Dec 15;515(4):367-94 PMID: 365237
  29. Differential polarized phase fluorometric investigations of diphenylhexatriene in lipid bilayers. Quantitation of hindered depolarizing rotations.
    Biochemistry. 1979 Feb 6;18(3):508-19 PMID: 420797
  30. Fluorescence lifetime and time-resolved polarization anisotropy studies of acyl chain order and dynamics in lipid bilayers.
    Biochemistry. 1981 May 12;20(10):2800-10 PMID: 6894699
  31. Effect of double bonds on the dynamic properties of the hydrocarbon region of lecithin bilayers.
    Biochemistry. 1981 Jul 21;20(15):4257-62 PMID: 7284325
  32. 1-[4-(Trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene: synthesis, fluorescence properties, and use as a fluorescence probe of lipid bilayers.
    Biochemistry. 1981 Dec 22;20(26):7333-8 PMID: 7326228
  33. Values for and significance of order parameters and "cone angles" of fluorophore rotation in lipid bilayers.
    Biochemistry. 1981 Dec 22;20(26):7338-45 PMID: 7326229
  34. Influence of cholesterol on water penetration into bilayers.
    Science. 1982 Apr 2;216(4541):65-7 PMID: 7063872
  35. A differential polarized phase fluorometric study of the effects of high hydrostatic pressure upon the fluidity of cellular membranes.
    Biochemistry. 1983 Jan 18;22(2):409-15 PMID: 6297548
  36. Differential polarized phase fluorometric studies of phospholipid bilayers under high hydrostatic pressure.
    Biochim Biophys Acta. 1983 Jul 27;732(2):359-71 PMID: 6871204
  37. A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution.
    Biophys J. 1983 Dec;44(3):315-24 PMID: 6661490
  38. Multifrequency phase and modulation fluorometry.
    Annu Rev Biophys Bioeng. 1984;13:105-24 PMID: 6378065
  39. The effects of pressure and cholesterol on rotational motions of perylene in lipid bilayers.
    Biochim Biophys Acta. 1985 Mar 14;813(2):253-65 PMID: 3970923
  40. Membrane structural domains. Resolution limits using diphenylhexatriene fluorescence decay.
    Biophys J. 1985 Aug;48(2):221-34 PMID: 4052559
  41. Cholesterol and the cell membrane.
    Biochim Biophys Acta. 1985 Dec 9;822(3-4):267-87 PMID: 3904832
  42. Steady-state fluorescence polarization data in membranes. Resolution into physical parameters by an extended Perrin equation for restricted rotation of fluorophores.
    Biochim Biophys Acta. 1986 Jan 16;854(1):38-44 PMID: 3942718
  43. Hydrostatic pressure induces hydrocarbon chain interdigitation in single-component phospholipid bilayers.
    Biochemistry. 1986 May 6;25(9):2591-6 PMID: 3718966
  44. Permeability of lipid bilayers to water and ionic solutes.
    Chem Phys Lipids. 1986 Jun-Jul;40(2-4):167-88 PMID: 2427233
  45. Area per molecule and distribution of water in fully hydrated dilauroylphosphatidylethanolamine bilayers.
    Biochemistry. 1986 Aug 26;25(17):4948-52 PMID: 3768325
  46. Structural changes in lipid bilayers and biological membranes caused hydrostatic pressure.
    Biochemistry. 1986 Nov 18;25(23):7484-8 PMID: 3801427
  47. Lateral proton conduction at a lipid/water interface. Effect of lipid nature and ionic content of the aqueous phase.
    Eur J Biochem. 1987 Jan 15;162(2):379-85 PMID: 3026808
  48. Resolvability of fluorescence lifetime distributions using phase fluorometry.
    Biophys J. 1987 Apr;51(4):587-96 PMID: 3580485
  49. A fluorescence study of dehydroergosterol in phosphatidylcholine bilayer vesicles.
    Biochemistry. 1987 May 5;26(9):2441-8 PMID: 3607026
  50. Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene in phospholipid vesicles.
    Biochemistry. 1987 Jun 30;26(13):3864-70 PMID: 3651418
  51. Equilibrium and dynamic structure of large, unilamellar, unsaturated acyl chain phosphatidylcholine vesicles. Higher order analysis of 1,6-diphenyl-1,3,5-hexatriene and 1-[4-(trimethylammonio)phenyl]- 6-phenyl-1,3,5-hexatriene anisotropy decay.
    Biochemistry. 1987 Aug 11;26(16):5113-20 PMID: 3663647
  52. Influence of cholesterol on equilibrium and dynamic bilayer structure of unsaturated acyl chain phosphatidylcholine vesicles as determined from higher order analysis of fluorescence anisotropy decay.
    Biochemistry. 1987 Aug 11;26(16):5121-6 PMID: 3663648
  53. Phase equilibria in the phosphatidylcholine-cholesterol system.
    Biochim Biophys Acta. 1987 Nov 27;905(1):162-72 PMID: 3676307
  54. Structure and properties of model membranes: new knowledge from high-pressure vibrational spectroscopy.
    Biochim Biophys Acta. 1988 Feb 24;947(1):139-71 PMID: 3278738
  55. Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane.
    Biochim Biophys Acta. 1988 Apr 22;939(3):485-92 PMID: 3355828
  56. High-resolution 13C NMR study of pressure effects on the main phase transition in L-alpha-dipalmitoyl phosphatidylcholine vesicles.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4115-7 PMID: 3380782
  57. Dynamic imaging of lateral diffusion by electron spin resonance and study of rotational dynamics in model membranes. Effect of cholesterol.
    Biophys J. 1989 Mar;55(3):537-50 PMID: 2539210
  58. A second generation global analysis program for the recovery of complex inhomogeneous fluorescence decay kinetics.
    Chem Phys Lipids. 1989 Jun;50(3-4):237-51 PMID: 2766430
  59. Influence of cholesterol on phospholipid bilayers phase domains as detected by Laurdan fluorescence.
    Biophys J. 1994 Jan;66(1):120-32 PMID: 8130331
  60. Hydrophobic barriers of lipid bilayer membranes formed by reduction of water penetration by alkyl chain unsaturation and cholesterol.
    Biochemistry. 1994 Jun 21;33(24):7670-81 PMID: 8011634
  61. Comparative differential scanning calorimetric and FTIR and 31P-NMR spectroscopic studies of the effects of cholesterol and androstenol on the thermotropic phase behavior and organization of phosphatidylcholine bilayers.
    Biophys J. 1994 Mar;66(3 Pt 1):741-52 PMID: 8011906
  62. Cholesterol modifies water concentration and dynamics in phospholipid bilayers: a fluorescence study using Laurdan probe.
    Biophys J. 1994 Mar;66(3 Pt 1):763-8 PMID: 8011908
  63. Evidence for regular distribution of sterols in liquid crystalline phosphatidylcholine bilayers.
    Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10069-73 PMID: 7937839
  64. New aspects of the interaction of cholesterol with dipalmitoylphosphatidylcholine bilayers as revealed by high-sensitivity differential scanning calorimetry.
    Biochim Biophys Acta. 1995 Mar 8;1234(1):90-8 PMID: 7880863
  65. Hydration and order in lipid bilayers.
    Biochemistry. 1995 May 9;34(18):6188-95 PMID: 7742324
  66. Temperature- and pressure-dependent phase behavior of monoacylglycerides monoolein and monoelaidin.
    Biophys J. 1995 Apr;68(4):1423-9 PMID: 7787028
  67. Abrupt modifications of phospholipid bilayer properties at critical cholesterol concentrations.
    Biophys J. 1995 May;68(5):1895-902 PMID: 7612832
  68. Evidence for a regular distribution of cholesterol in phospholipid bilayers from diphenylhexatriene fluorescence.
    Biophys J. 1995 May;68(5):1944-51 PMID: 7612836
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(97)78773-3
Published
1997-03-00
Pages
1264-77
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1184509
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(97)78773-3 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2