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PMID: 9121469 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Complex formation between p53 and replication protein A inhibits the sequence-specific DNA binding of p53 and is regulated by single-stranded DNA.

Molecular and cellular biology ·Vol. 17 ·No. 4 ·1997-04-00 ·Pages 2194-201

Miller SD, Moses K, Jayaraman L, Prives C

Abstract

Human replication protein A (RP-A) (also known as human single-stranded DNA binding protein, or HSSB) is a multisubunit complex involved in both DNA replication and repair. Potentially important to both these functions, it is also capable of complex formation with the tumor suppressor protein p53. Here we show that although p53 is unable to prevent RP-A from associating with a range of single-stranded DNAs in solution, RP-A is able to strongly inhibit p53 from functioning as a sequence-specific DNA binding protein when the two proteins are complexed. This inhibition, in turn, can be regulated by the presence of various lengths of single-stranded DNAs, as RP-A, when bound to these single-stranded DNAs, is unable to interact with p53. Interestingly, the lengths of single-stranded DNA capable of relieving complex formation between the two proteins represent forms that might be introduced through repair and replicative events. Increasing p53 concentrations can also overcome the inhibition by steady-state levels of RP-A, potentially mimicking cellular points of balance. Finally, it has been shown previously that p53 can itself be stimulated for site-specific DNA binding when complexed through the C terminus with short single strands of DNA, and here we show that p53 stays bound to these short strands even after binding a physiologically relevant site. These results identify a potential dual role for single-stranded DNA in the regulation of DNA binding by p53 and give insights into the p53 response to DNA damage.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cell Line Cyclin-Dependent Kinase Inhibitor p21 Cyclins/metabolism DNA, Single-Stranded/genetics,metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Humans Macromolecular Substances Models, Biological Molecular Sequence Data Protein Binding Replication Protein A Tumor Suppressor Protein p53/chemistry,genetics,metabolism
Chemicals
CDKN1A protein, human Cyclin-Dependent Kinase Inhibitor p21 Cyclins DNA, Single-Stranded DNA-Binding Proteins Macromolecular Substances RPA1 protein, human Replication Protein A Tumor Suppressor Protein p53
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miller S D
Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Moses K
Jayaraman L
Prives C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.4.2194
Published
1997-04-00
Pages
2194-201
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232068
Subset
IM
Grants
NCI NIH HHS · CA26905 · United States
NCI NIH HHS · CA58316 · United States
External Links
PubMed source DOI: 10.1128/MCB.17.4.2194 PMC Full Text View journal details
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