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PMID: 91169 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Reverse transcriptase pauses at N2-methylguanine during in vitro transcription of Escherichia coli 16S ribosomal RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 8 ·1979-08-00 ·Pages 3751-4

Youvan DC, Hearst JE

Abstract

A restriction fragment strand complementary to a sequence near the 3' end of Escherichia coli 16S rRNA has been used to prime reverse transcriptase (avian myeloblastosis virus RNA-directed DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7). In addition to transcripts that were extended to the 5' end of the RNA, two major transcription intermediates were observed. These discrete-sized cDNA intermediates are the result of a kinetic barrier imposed by monomethylation of the amino group on guanine that participates in base-pairing. Both major transcription intermediates correspond to attenuation at the known positions of N2-methylguanine (m2G) in the rRNA sequence. The relaxation time for elongation of the cDNA through m2G is approximately 3 min. No other major kinetic pauses were observed in the 1340 bases transcribed.

MeSH Terms
Base Sequence Escherichia coli Guanine Nucleotides/metabolism Kinetics RNA, Bacterial/metabolism RNA, Ribosomal/metabolism RNA-Directed DNA Polymerase/metabolism Substrate Specificity Templates, Genetic Transcription, Genetic
Chemicals
Guanine Nucleotides RNA, Bacterial RNA, Ribosomal RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Youvan D C
Hearst J E
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18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.8.3751
Published
1979-08-00
Pages
3751-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383911
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.8.3751 PMC Full Text View journal details
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