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PMID: 9116279 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Lineage- and stage-specific expression of runt box polypeptides in primitive and definitive hematopoiesis.

Blood ·Vol. 89 ·No. 7 ·1997-04-01 ·Pages 2359-68

Corsetti MT, Calabi F

Abstract

Translocations involving the human CBFA2 locus have been associated with leukemia. This gene, originally named AML1, is a human homologue of the Drosophila gene runt that controls early events in fly embryogenesis. To clarify the role of mammalian runt products in normal and leukemic hematopoiesis, we have studied their pattern of expression in mouse hematopoietic tissues in the adult and during ontogeny using an anti-runt box antiserum. In the adult bone marrow, we found expression of runt polypeptides in differentiating myeloid cells and in B lymphocytes. Within the erythroid lineage, runt expression is biphasic, clearly present in the erythroblasts of early blood islands and of the fetal liver, but absent in the adult. Biochemical analysis by Western blotting of fetal and adult hematopoietic populations shows several runt isoforms. At least one of them appears to be myeloid specific.

MeSH Terms

Age Factors Animals Blotting, Western Cell Lineage Chromosomes, Human, Pair 21/genetics,ultrastructure Core Binding Factor Alpha 2 Subunit DNA-Binding Proteins/genetics Drosophila Proteins Drosophila melanogaster/embryology,genetics Erythroblasts/metabolism Fluorescent Antibody Technique, Indirect Gene Expression Regulation Gene Expression Regulation, Leukemic Hematopoiesis Hematopoietic System/cytology,embryology,growth & development Humans Immune Sera Leukemia/genetics,pathology Leukemia, Myeloid/genetics,pathology Liver/cytology,embryology Mice Neoplasm Proteins/biosynthesis,genetics Nuclear Proteins Organ Specificity Peptide Fragments/genetics,immunology Proto-Oncogene Proteins Rabbits Recombinant Fusion Proteins/biosynthesis Species Specificity Transcription Factors/biosynthesis,genetics Translocation, Genetic Yolk Sac/cytology

Chemicals

Core Binding Factor Alpha 2 Subunit DNA-Binding Proteins Drosophila Proteins Immune Sera Neoplasm Proteins Nuclear Proteins Peptide Fragments Proto-Oncogene Proteins RUNX1 protein, human Recombinant Fusion Proteins Runx1 protein, mouse Transcription Factors run protein, Drosophila

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Corsetti M T

Developmental Biology Unit, Division of Cell and Molecular Biology, Institute of Child Health, London, UK.

Calabi F

Article Info

Journal

Blood

Abbr.

Blood

ISSN

0006-4971

Published

1997-04-01

Pages

2359-68

Language

English

Region

United States

NLM ID

7603509

Subset

External Links

PubMed source View journal details

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Lineage- and stage-specific expression of runt box polypeptides in primitive and definitive hematopoiesis.

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