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PMID: 9114008 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of a putative human serine/threonine protein kinase transcriptionally modified during anisotonic and isotonic alterations of cell volume.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 9 ·1997-04-29 ·Pages 4440-5

Waldegger S, Barth P, Raber G, Lang F

Abstract

Hepatic metabolism and gene expression are among other regulatory mechanisms controlled by the cellular hydration state, which changes rapidly in response to anisotonicity, concentrative substrate uptake, oxidative stress, and under the influence of hormones such as insulin and glucagon. Differential screening for cell volume sensitive transcripts in a human hepatoma cell line revealed a gene for a putative serine/threonine kinase, h-sgk, which has 98% sequence identity to a serum- and glucocorticoid regulated kinase, sgk, cloned from a rat mammary tumor cell line. h-sgk transcript levels were strongly altered during anisotonic and isotonic cell volume changes. Within 30 min h-sgk RNA was, independent of de novo protein synthesis, induced upon cell shrinkage and, due to a complete stop in h-sgk transcription, reduced upon cell swelling. Comparable changes of sgk transcript levels were observed in a renal epithelial cell line. h-sgk mRNA was detected in all human tissues tested, with the highest levels in pancreas, liver, and heart. The putative serine/threonine protein kinase h-sgk may provide a functional link between the cellular hydration state and metabolic control.

MeSH Terms
Animals Cell Size/physiology Cloning, Molecular Dogs Epithelial Cells Epithelium/physiology Gene Expression Regulation, Enzymologic Genes, Regulator Hypertonic Solutions Hypotonic Solutions Immediate-Early Proteins Isotonic Solutions Kidney/cytology,physiology Liver/cytology,physiology Molecular Sequence Data Nuclear Proteins Osmotic Pressure Protein Serine-Threonine Kinases/genetics Sequence Analysis, DNA Transcription, Genetic Tumor Cells, Cultured Water/metabolism
Chemicals
Hypertonic Solutions Hypotonic Solutions Immediate-Early Proteins Isotonic Solutions Nuclear Proteins Water Protein Serine-Threonine Kinases serum-glucocorticoid regulated kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Waldegger S
Physiologisches Institut I der Eberhard-Karls-Universität, D-72076 Tübingen, Germany.
Barth P
Raber G
Lang F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.9.4440
Published
1997-04-29
Pages
4440-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20741
Subset
IM
Databases
GENBANK
Y10032
External Links
PubMed source DOI: 10.1073/pnas.94.9.4440 PMC Full Text View journal details
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