Home LiteratureArticle Details
PMID: 9101407 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Monovalent ion selectivity sequences of the rat connexin43 gap junction channel.

The Journal of general physiology ·Vol. 109 ·No. 4 ·1997-04-00 ·Pages 491-507

Wang HZ, Veenstra RD

Abstract

The relative permeability sequences of the rat connexin 43 (rCx43) gap junction channel to seven cations and chloride were examined by double whole cell patch clamp recording of single gap junction channel currents in rCx43 transfected neuroblastoma 2A (N2A) cell pairs. The measured maximal single channel slope conductances (gammaj, in pS) of the junctional current-voltage relationships in 115 mM XCI were RbC1 (103) > or = CsC1 (102) > KC1 (97) > NaC1 (79) > or = LiC1 (78) > TMAC1 (65) > TEAC1 (53) and for 115 mM KY were KBr (105) > KC1 (97) > Kacetate (77) > Kglutamate (61). The single channel conductance- aqueous mobility relationships for the test cations and anions were linear. However, the predicted minimum anionic and cationic conductances of these plots did not accurately predict the rCx43 channel conductance in 115 mM KC1. Instead, the conductance of the rCx43 channel in 115 mM KC1 was accurately predicted from cationic and anionic conductance-mobility plots by applying a mobility scaling factor Dx/Do, which depends upon the relative radii of the permeant ions to an estimated pore radius. Relative permeabilities were determined for all of the monovalent catious and anions tested from asymmetric salt reversal potential measurements and the Goldman-Hodgkin-Katz voltage equation. These experiments estimate the relative chloride to potassium permeability to be 0.13. The relationship between the relative cation permeability and hydrated radius was modeled using the hydrodynamic equation assuming a pore radius of 6.3 +/- 0.4 A. Our data quantitatively demonstrate that the rCx43 gap junction channel is permeable to monovalent atomic and organic cations and anions and the relative permeability sequences are consistent with an Eisenman sequence II or I, respectively. These predictions about the rCx43 channel pore provide a useful basis for future investigations into the structural determinants of the conductance and permeability properties of the connexin channel pore.

MeSH Terms
Animals Cations/metabolism Connexin 43/metabolism Diffusion Electric Stimulation Electrophysiology Gap Junctions/metabolism Ion Channels/metabolism Membrane Potentials/physiology Mice Models, Biological Osmolar Concentration Patch-Clamp Techniques Permeability Porosity Rats Tumor Cells, Cultured
Chemicals
Cations Connexin 43 Ion Channels
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang H Z
Department of Pharmacology, SUNY Health Science Center at Syracuse, 13210, USA.
Veenstra R D
References (34)
34 references, click to expand
  1. Permeability of the cell-to-cell membrane channels in mammalian cell juncton.
    Science. 1979 Jul 27;205(4404):404-7 PMID: 377490
  2. Human connexin43 gap junction channels. Regulation of unitary conductances by phosphorylation.
    Circ Res. 1994 Jun;74(6):1050-7 PMID: 7514508
  3. The permeability of the endplate channel to organic cations in frog muscle.
    J Gen Physiol. 1980 May;75(5):469-92 PMID: 6247422
  4. Evidence for fixed charge in the nexus.
    Nature. 1980 May 8;285(5760):101-2 PMID: 6246436
  5. Mechanism of anion-cation selectivity of amphotericin B channels.
    J Membr Biol. 1986;90(1):13-20 PMID: 2422383
  6. Single-channel currents of an intercellular junction.
    Nature. 1985 Sep 26-Oct 2;317(6035):331-5 PMID: 2413362
  7. Gap junction structures. V. Structural chemistry inferred from X-ray diffraction measurements on sucrose accessibility and trypsin susceptibility.
    J Mol Biol. 1984 Apr 15;174(3):449-81 PMID: 6716484
  8. Sodium and calcium channels in bovine chromaffin cells.
    J Physiol. 1982 Oct;331:599-635 PMID: 6296372
  9. Diameter of the cell-to-cell junctional membrane channels as probed with neutral molecules.
    Science. 1981 Jul 31;213(4507):551-3 PMID: 7244653
  10. Calcium channel selectivity for divalent and monovalent cations. Voltage and concentration dependence of single channel current in ventricular heart cells.
    J Gen Physiol. 1986 Sep;88(3):293-319 PMID: 2428919
  11. Ionic selectivity revisited: the role of kinetic and equilibrium processes in ion permeation through channels.
    J Membr Biol. 1983;76(3):197-225 PMID: 6100862
  12. Formation of gap junctions by expression of connexins in Xenopus oocyte pairs.
    Cell. 1989 Apr 7;57(1):145-55 PMID: 2467743
  13. Formation of hybrid cell-cell channels.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5380-4 PMID: 2546155
  14. Patch clamp recordings from membranes which contain gap junction channels.
    Biophys J. 1989 Sep;56(3):579-93 PMID: 2477073
  15. Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA.
    J Cell Biol. 1989 Dec;109(6 Pt 2):3391-401 PMID: 2557354
  16. Molecular characterization and functional expression of the human cardiac gap junction channel.
    J Cell Biol. 1990 Aug;111(2):589-98 PMID: 1696265
  17. Connexin family of gap junction proteins.
    J Membr Biol. 1990 Jul;116(3):187-94 PMID: 2167375
  18. General continuum theory for multiion channel. II. Application to acetylcholine channel.
    Biophys J. 1991 Feb;59(2):278-88 PMID: 1706950
  19. Functional analysis of human cardiac gap junction channel mutants.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):3525-9 PMID: 1850831
  20. Multichannel recordings from membranes which contain gap junctions.
    Biophys J. 1992 Jan;61(1):216-27 PMID: 1371704
  21. Threonine in the selectivity filter of the acetylcholine receptor channel.
    Biophys J. 1992 Apr;62(1):196-205; discussion 205-8 PMID: 1376167
  22. Multiple connexins confer distinct regulatory and conductance properties of gap junctions in developing heart.
    Circ Res. 1992 Nov;71(5):1277-83 PMID: 1382884
  23. Chick connexin-56, a novel lens gap junction protein. Molecular cloning and functional expression.
    J Biol Chem. 1993 Jan 5;268(1):706-12 PMID: 7678009
  24. Cardiac gap junctions: three distinct single channel conductances and their modulation by phosphorylating treatments.
    Pflugers Arch. 1992 Nov;422(2):198-200 PMID: 1283218
  25. Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein.
    J Clin Invest. 1993 Mar;91(3):997-1004 PMID: 7680674
  26. Fluorescent properties of rat cardiac trabeculae microinjected with fura-2 salt.
    Am J Physiol. 1993 Apr;264(4 Pt 2):H1098-110 PMID: 8476086
  27. Zero-current potentials in a large membrane channel: a simple theory accounts for complex behavior.
    Biophys J. 1993 Sep;65(3):1093-100 PMID: 7694668
  28. Multichannel recordings from membranes which contain gap junctions. II. Substates and conductance shifts.
    Biophys J. 1993 Oct;65(4):1387-95 PMID: 7506063
  29. Monovalent cation permeation through the connexin40 gap junction channel. Cs, Rb, K, Na, Li, TEA, TMA, TBA, and effects of anions Br, Cl, F, acetate, aspartate, glutamate, and NO3.
    J Gen Physiol. 1997 Apr;109(4):509-22 PMID: 9101408
  30. Selectivity of connexin-specific gap junctions does not correlate with channel conductance.
    Circ Res. 1995 Dec;77(6):1156-65 PMID: 7586229
  31. A multi-ion permeation mechanism in neuronal background chloride channels.
    J Gen Physiol. 1994 Oct;104(4):725-46 PMID: 7836939
  32. Connexin37 forms high conductance gap junction channels with subconductance state activity and selective dye and ionic permeabilities.
    Biophys J. 1994 Jun;66(6):1915-28 PMID: 7521227
  33. Selective dye and ionic permeability of gap junction channels formed by connexin45.
    Circ Res. 1994 Sep;75(3):483-90 PMID: 7520372
  34. Molecular cloning of cDNA for rat liver gap junction protein.
    J Cell Biol. 1986 Jul;103(1):123-34 PMID: 3013898
Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.109.4.491
Published
1997-04-00
Pages
491-507
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2219435
Subset
IM
Grants
NHLBI NIH HHS · R01 HL042220 · United States
NHLBI NIH HHS · HL-45466 · United States
NHLBI NIH HHS · HL-42220 · United States
External Links
PubMed source DOI: 10.1085/jgp.109.4.491 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2