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PMID: 909775 Published · ppublish English Journal Article

Chromatin very small angle neutron scattering: further evidence for a 30 nm diameter super coil in dilute solutions.

Nucleic acids research ·Vol. 4 ·No. 7 ·1977-07-00 ·Pages 2275-82

Bram S, Baudy P, Lepault J, Hermann D

Abstract

Intact chromatin, chromatin minus histone H1, and nuclease digestion fragments have been studied by very small angle neutron scattering. The results are not consistent with a straight chain of nucleosomes and require the presence of a higher order coiling in monovalent salt solutions. The data are interpretable by a structure having a cross section radius of gryration of 8.5 +/- 1 nm, which suggests an outer diameter for a coil of nucleosomes of 27 +/- 3 nm.

MeSH Terms
Animals Cattle Chromatin/ultrastructure DNA Deoxyribonucleases Histones Neutrons Nucleic Acid Conformation Rats Scattering, Radiation
Chemicals
Chromatin Histones DNA Deoxyribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bram S
Baudy P
Lepault J
Hermann D
References (15)
15 references, click to expand
  1. Chromatin subunit small angle neutron scattering: a DNA rich coil surrounds a protein-DNA core.
    Biochem Biophys Res Commun. 1976 Sep 7;72(1):176-83 PMID: 985465
  2. Quasielastic light scattering by biopolymers. Conformation of chromatin multimers.
    Biochem Biophys Res Commun. 1976 Nov 22;73(2):224-32 PMID: 999708
  3. Solenoidal model for superstructure in chromatin.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1897-901 PMID: 1064861
  4. A double coil chromatin sub-unit model.
    Biochimie. 1975 Nov-Dec;57(11-12):1301-6 PMID: 1222128
  5. The mass per unit length of chromatin by low-angle x-ray scattering.
    FEBS Lett. 1976 Apr 15;64(1):89-91 PMID: 1269768
  6. Properties of nuclease-resistant fragments of calf thymus chromatin.
    J Biol Chem. 1973 Feb 10;248(3):1080-3 PMID: 4684705
  7. Chromatin structure; oligomers of the histones.
    Science. 1974 May 24;184(4139):865-8 PMID: 4825888
  8. On the structure of nucleohistone.
    J Mol Biol. 1971 Feb 14;55(3):325-36 PMID: 5102527
  9. Organisation of subunits in chromatin.
    Nucleic Acids Res. 1976 Jul;3(7):1739-46 PMID: 967672
  10. Quaternary structure of chromatin.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1043-5 PMID: 1055363
  11. Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.
    Cell. 1975 Apr;4(4):281-300 PMID: 1122558
  12. Chromatin model calculations: Arrays of spherical nu bodies.
    Nucleic Acids Res. 1976 Jan;3(1):89-100 PMID: 1250708
  13. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.
    Biochem Biophys Res Commun. 1973 May 15;52(2):504-10 PMID: 4711166
  14. An electrophoretic comparison of vertebrate histones.
    J Biol Chem. 1971 Jul 10;246(13):4206-15 PMID: 5090040
  15. Preparation of native chromatin and damage caused by shearing.
    Science. 1975 Mar 28;187(4182):1203-6 PMID: 17754290
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.7.2275
Published
1977-07-00
Pages
2275-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342565
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.7.2275 PMC Full Text View journal details
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