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PMID: 9096341 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nuclear and cytoplasmic localizations of the adenomatous polyposis coli protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 7 ·1997-04-01 ·Pages 3034-9

Neufeld KL, White RL

Abstract

Mutation of the adenomatous polyposis coli (APC) gene is an early step in the initiation of colon cancer. Because the distribution pattern of a protein within the cell can provide important clues as to function, we have used a combination of immunofluorescence microscopy and biochemical fractionation to determine the location of APC protein in epithelial cells. Immunofluorescence microscopy placed full-length APC protein in both the nucleus and the cytoplasm. The nuclear APC protein was concentrated in discrete subnuclear regions, including nucleoli, whereas the cytoplasmic APC protein concentrated at the leading edge of migrating cells. Colocalization of APC protein with rRNA confirmed a nucleolar localization. These immunocytochemical findings have been supported by cell fractionation, which demonstrated that full-length APC protein was located in both the membrane/cytoskeletal and the nuclear fractions.

MeSH Terms
Adenomatous Polyposis Coli Protein Biological Transport Cell Line, Transformed Cell Nucleus/metabolism Colonic Neoplasms/metabolism,pathology Cytoplasm/metabolism Cytoskeletal Proteins/metabolism Epithelium/metabolism Genes, APC Microscopy, Fluorescence Tumor Cells, Cultured
Chemicals
Adenomatous Polyposis Coli Protein Cytoskeletal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Neufeld K L
Huntsman Cancer Institute, University of Utah, Salt Lake City 84112, USA.
White R L
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.7.3034
Published
1997-04-01
Pages
3034-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20317
Subset
IM
Grants
NCI NIH HHS · P30 CA042014 · United States
NCI NIH HHS · T32 CA009602 · United States
NCI NIH HHS · CA09602 · United States
NCI NIH HHS · P30 CA42014-10 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.7.3034 PMC Full Text View journal details
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